Semisynthesis of a Glycosylphosphatidylinositol-Anchored Prion Protein

被引：78

作者：

Becker, Christian F. W. ^{[1
]}

Liu, Xinyu ^{[2
]}

Olschewski, Diana ^{[1
]}

Castelli, Riccardo ^{[2
]}

Seidel, Ralf ^{[1
]}

Seeberger, Peter H. ^{[2
]}

机构：

[1] Max Planck Inst Mol Physiol, Dept Phys Biochem, D-44227 Dortmund, Germany

[2] Swiss Fed Inst Technol, Organ Chem Lab, CH-8093 Zurich, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 43期

基金：

瑞士国家科学基金会;

关键词：

glycosylphosphatidylinositol; native chemical ligation; protein semisynthesis; proteins; total synthesis;

D O I：

10.1002/anie.200802161

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Pinning down the role of the anchor: The chemical synthesis of a cysteine-modified glycosylphosphatidylinositol (GPI) anchor provides access to homogeneous GPI-anchored prion protein through expressed protein ligation (see scheme). By this method, it should be possible to investigate the influence of the complex posttranslational GPI modification on protein structure and function. (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8215 / 8219

页数：5

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