Gordonia namibiensis sp nov., a novel nitrile metabolising actinomycete recovered from an African sand

被引:20
作者
Brandao, PFB [1 ]
Maldonado, LA
Ward, AC
Bull, AT
Goodfellow, M
机构
[1] Univ Newcastle Upon Tyne, Dept Agr & Environm Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[2] Univ Kent, Res Sch Biosci, Canterbury, Kent, England
关键词
Gordonia; polyphasic taxonomy; mycolic acids; 16S rDNA sequencing;
D O I
10.1078/0723-2020-00074
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A polyphasic approach was used to establish the taxonomic position of two actinomycetes isolated from a Namibian soil and shown to utilise nitrile compounds as growth substrates. The organisms, strains NAM-BN063A(inverted perpendicular) and NAM-BN063B, had chemical and morphological properties consistent with their assignment to the genus Gordonia. Direct 16S rRNA sequencing studies confirmed the taxonomic position of the strains following the generation of phylogenetic trees using four different algorithms. The strains consistently formed a distinct phylogenetic line within the evolutionary radiation occupied by gordoniae and were most closely related to Gordonia rubropertincta DSM 43197(inverted perpendicular). DNA:DNA relatedness studies indicated that the two organisms belonged to a genomic species that was readily distinguished from G. rubropertincta. The unique phenotypic profile of the strains sharply separated them from representatives of all of the validly described species of Gordonia. The combination of genotypic and phenotypic data indicates that the two strains should be classified in the genus Gordonia as a new species. The name proposed for this taxon is Gordonia namibiensis, the type strain is NAM-BN063A(inverted perpendicular) (= DSM 44568(inverted perpendicular) = NCIMB 13780(inverted perpendicular)).
引用
收藏
页码:510 / 515
页数:6
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