Purification and characterization of novel extracellular endopolygalacturonases from a deep-sea yeast, Cryptococcus sp N6, isolated from the Japan Trench

被引：11

作者：

Miura, T

Abe, F

Inoue, A

Usami, R

Horikoshi, K

机构：

[1] Japan Marine Sci & Technol Ctr, DEEPSTAR Grp, Yokosuka, Kanagawa 2370061, Japan

[2] Toyo Univ, Fac Engn, Dept Appl Chem, Kawagoe, Saitama 3500815, Japan

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 21期

关键词：

copper-tolerant yeast; deep-sea yeast; endopolygalacturonase; glycoprotein; Cryptococcus;

D O I：

10.1023/A:1012488115482

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two novel endo-type polygalacturonases (PGase), with molecular weights of 36 kDa and 40 kDa (named p36 and p40, respectively), were purified from the supernatant of the culture medium of a deep-sea yeast, strain N6, isolated from the Japan Trench. The N-terminal 20 amino acids of p36 and p40 were identical, and the sequence homology was 47.4% in comparison with the PGase of Fusarium moniliforme. A treatment of p40 with glycopeptidase F reduced the molecular weight to 36 kDa, suggesting that p40 possessed N-acetylglucosamines on its asparagine residues and p40 might be matured by glycosylation of p36.

引用

页码：1735 / 1739

页数：5