Telomerase activity distinguishes between neuroblastomas with good and poor prognosis

被引:48
作者
Poremba, C
Willenbring, H
Hero, B
Christiansen, H
Schäfer, KL
Brinkschmidt, C
Jürgens, H
Böcker, W
Dockhorn-Dworniczak, B
机构
[1] Univ Munster, Gerhard Domagk Inst Pathol, D-48149 Munster, Germany
[2] Univ Munster, Dept Pediat, D-48149 Munster, Germany
[3] Univ Munster, Dept Pediat Hematol & Oncol, D-48149 Munster, Germany
[4] Univ Cologne, Dept Pediat Hematol & Oncol, D-5000 Cologne 41, Germany
[5] Univ Marburg, Dept Pediat Hematol & Oncol, D-35032 Marburg, Germany
关键词
neuroblastoma; prognosis; telomerase activity;
D O I
10.1023/A:1008333500733
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Treatment of neuroblastoma has remained a major challenge in pediatric oncology because the assessment of the individual prognosis, particularly in disseminated disease is still obscure. Previous studies have correlated clinical outcome with activity levels of telomerase, a cellular reverse transcriptase which has been detected in the majority of human malignant tumors. Patients and methods: In this blind-trial study, a non-radioactive telomeric repeat amplification protocol (TRAP) with an internal telomerase-assay standard was used on an automated laser fluorescence sequencer for the detection and semiquantitative analysis of telomerase activity (TA) in 67 neuroblastomas of all clinical stages from the German Neuroblastoma Trial and 2 ganglioneuromas. TA levels were correlated with event-free and overall survival rates and established prognostic markers such as MYCN. Results: TA was present in 14 of 69 (20%) samples, including 3 of 22 stage IVS, 8 of 14 stage IV, 1 of 10 stage III, 1 of 7 stage II and 1 of 14 stage I neuroblastomas and 0 of 2 ganglioneuromas. We found a strong statistical correlation between the presence of TA and poor clinical prognosis with regard to all tumor stages. Multivariate analysis revealed TA as an independent prognostic marker. In particular, the analysis of TA in IVS neuroblastomas distinguished two different prognostic groups. Conclusions: Our data suggest that TA is an independent prognostic marker in neuroblastoma which, in combination with other markers such as MYCN, may proof useful in assessing the individual patient's prognosis.
引用
收藏
页码:715 / 721
页数:7
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