The use of retroviruses as pharmaceutical tools for target discovery and validation in the field of functional genomics

被引：22

作者：

Lorens, JB ^{[1
]}

Sousa, C ^{[1
]}

Bennett, MK ^{[1
]}

Molineaux, SM ^{[1
]}

Payan, DG ^{[1
]}

机构：

[1] Rigel Inc, S San Francisco, CA 94080 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 06期

关键词：

D O I：

10.1016/S0958-1669(01)00269-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Retrovirally mediated functional genomics enables identification of physiologically relevant cellular therapeutic targets. Unique properties of retroviruses make them ideal tools for the introduction of large and diverse libraries of potential genetic effectors to a variety of cell types. The identification and recovery of intracellular library elements responsible for altered disease responses establishes a direct basis for pharmaceutical development. Recent innovations in retroviral infection efficiency and expression control have broadened application of the methodology to include libraries of mutagenized cDNAs, peptides and ribozyme genetic effectors.

引用

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页码：613 / 621

页数：9

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