Detecting biomarkers with microdroplet technology

被引：96

作者：

Taly, Valerie ^{[1
,2
]}

Pekin, Deniz ^{[2
]}

El Abed, Abdel ^{[1
]}

Laurent-Puig, Pierre ^{[1
]}

机构：

[1] Univ Paris Sorbonne Cite, INSERM, UMR S775, F-75270 Paris 06, France

[2] Univ Strasbourg, ISIS, CNRS, UMR 7006, F-67083 Strasbourg, France

来源：

TRENDS IN MOLECULAR MEDICINE | 2012年 / 18卷 / 07期

关键词：

POLYMERASE-CHAIN-REACTION; COLORECTAL-CANCER PATIENTS; OVARIAN SEROUS CARCINOMA; SINGLE DNA-MOLECULES; IN-OIL EMULSION; HIGH-THROUGHPUT; DIGITAL PCR; PICOLITER DROPLETS; COPY-NUMBER; REAL-TIME;

D O I：

10.1016/j.molmed.2012.05.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The efficient analysis and noninvasive detection of molecules such as DNA, mRNA, and miRNA for clinical diagnostics requires sensitive, high-throughput methods. By segregating individual sequences within separate compartments, digital procedures allow identification of very rare sequences. These procedures are based on the limiting dilution of biological samples in individual compartments such as droplets of a water-in-oil emulsion, and relies on the discrete counting of a given event, providing an absolute value and quantitative data. Coupled with microfluidic systems, digital procedures could become an essential diagnostic tool for the study of diseases as well as patient management.

引用

页码：405 / 416

页数：12

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