Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei

被引：48

作者：

de Faria, FP

Te'o, VSJ

Bergquist, PL

Azevedo, MO

Nevalainen, KMH ^{[1
]}

机构：

[1] Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia

[2] FAENQUIL, Dept Biotecnol, Lorena, SP, Brazil

[3] Univ Auckland, Sch Med, Div Mol Med, Auckland, New Zealand

[4] Univ Brasilia, IB, Dept Biol Celular, Brasilia, DF, Brazil

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2002年 / 34卷 / 02期

关键词：

D O I：

10.1046/j.1472-765x.2002.01057.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To express a gene encoding a heterologous fungal xylanase in Trichoderma reesei. Methods and Results: Humicola grisea xylanase 2 (xyn2) cDNA was expressed in Trichoderma reesei under the main cellobiohydrolase I (cbh1) promoter (i) as a fusion to the cellobiohydrolase I (CBHI) secretion signal and (ii) the mature CBHI core-linker. The recombinant xylanase (HXYN2) was secreted into the cultivation medium and processed in a similar fashion to the endogenous T. reesei xylanases, resulting in an active enzyme. Conclusions, Significance and Impact of the Study: HXYN2 was successfully processed in T. reesei. Composition of the culture medium affected the HXYN2 yields, favouring Avicel-lactose as a carbon source. Best yields (about 0.5 g l(-1)) in shake flask cultivations were obtained from a transformant where xyn2 was fused directly to the CBHI secretion signal.

引用

页码：119 / 123

页数：5

共 23 条

[21] USE OF CONGO RED POLYSACCHARIDE INTERACTIONS IN ENUMERATION AND CHARACTERIZATION OF CELLULOLYTIC BACTERIA FROM THE BOVINE RUMEN [J].