The yeast Saccharomyces cerevisiae does not sequester chloride but can express a functional mammalian chloride channel

被引:12
作者
Coury, LA
McGeoch, JEM
Guidotti, G
Brodsky, JL [1 ]
机构
[1] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA
[2] Univ Pittsburgh, Med Ctr, Dept Med, Renal Electrolyte Div, Pittsburgh, PA 15213 USA
[3] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
关键词
yeast; chloride transport; pI(Cln); heterologous expression;
D O I
10.1111/j.1574-6968.1999.tb08745.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chloride uptake into yeast was measured as a function of pH, A small amount of uptake was seen at pH values of 3.0 and 4.0; at pH 6.0 chloride uptake was substantially less than the uptake of phosphate and rubidium. Because chloride uptake is inefficient, we expressed the putative mammalian chloride channel, pI(Cln), in yeast and observed a chloride-selective current when total membrane protein was reconstituted into lipid bilayers. The current was inhibited by a specific chloride channel blocker, 5-nitro-2-(3-phenylpropylamino)-benzoic acid. These results suggest that yeast may serve as a means to characterize chloride channels from other organisms. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:327 / 332
页数:6
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