Structural organization of the human flavin-containing monooxygenase 3 gene (FMO3), the favored candidate for fish-odor syndrome, determined directly from genomic DNA

被引:46
作者
Dolphin, CT
Riley, JH
Smith, RL
Shephard, EA
Phillips, IR
机构
[1] UNIV LONDON, UNIV LONDON QUEEN MARY & WESTFIELD COLL, DEPT BIOCHEM, LONDON E1 4NS, ENGLAND
[2] GLAXO WELLCOME MED RES CTR, GENOM UNIT, STEVENAGE SG1 2NY, HERTS, ENGLAND
[3] UNIV LONDON, ST MARYS HOSP, SCH MED, DEPT PHARMACOL & TOXICOL, LONDON W2 1PG, ENGLAND
[4] UNIV LONDON UNIV COLL, DEPT BIOCHEM & MOL BIOL, LONDON WC1E 6BT, ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1006/geno.1997.5031
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The inherited metabolic disorder trimethylaminuria (fish-odor syndrome) is associated with defective hepatic N-oxidation of dietary-derived trimethylamine catalyzed by flavin-containing monooxygenase (FMO). As FMO3 encodes the major form of FMO expressed in adult human liver, it represents the best candidate gene for the disorder. The structural organization of FMO3 was determined by sequencing the products of exon-to-exon and vectorette PCR, the latter through the use of vectorette libraries constructed directly from genomic DNA. The gene contains one noncoding and eight coding exons. Knowledge of the exon/intron organization of the human FMO3 gene enabled each of the coding exons of the gene, together with their associated flanking intron sequences, to be amplified from genomic DNA and will thus facilitate the identification of mutations in FMO3 in families affected with fish-odor syndrome. (C) 1997 Academic Press.
引用
收藏
页码:260 / 267
页数:8
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