A unified procedure for the correction of liquid junction potentials in patch clamp experiments on endo- and plasma membranes

被引：17

作者：

Amtmann, A

Sanders, D

机构：

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 1997年 / 48卷

关键词：

patch clamp; liquid junction potentials; salt bridges; membranes; ion channels;

D O I：

10.1093/jxb/48.Special_Issue.361

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Correct determination of absolute values and polarities of liquid junction potentials (LJPs) is essential in patch clamp experiments for the estimation of ionic selectivities and activation voltages of ion channels. A simple approach for the correction of membrane voltages for LJPs has been developed. The method covers all combinations of LJPs between the solutions of bath, patch pipette and reference salt bridge and is applicable to all patch configurations on plasma membranes and endomembranes.

引用

页码：361 / 364

页数：4

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