MicroRNA-27a promotes myoblast proliferation by targeting myostatin

被引：179

作者：

Huang, Zhiqing ^{[1
]}

Chen, Xiaoling ^{[1
]}

Yu, Bing ^{[1
]}

He, Jun ^{[1
]}

Chen, Daiwen ^{[1
]}

机构：

[1] Sichuan Agr Univ, Inst Anim Nutr, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Yaan 625014, Sichuan, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 423卷 / 02期

关键词：

MicroRNA; miR-27a; Myoblast proliferation; Myostatin; NEGATIVE REGULATOR; DIFFERENTIATION; EXPRESSION; GENE; MIR-206; GROWTH;

D O I：

10.1016/j.bbrc.2012.05.106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play critical roles in skeletal muscle development as well as in regulation of muscle cell proliferation and differentiation. However, the role of miRNAs in myoblast proliferation remains poorly understood. Here we found that the expression of miR-27a was increased during proliferation of C2C12 myoblasts. Moreover, overexpression of miR-27a in C2C12 cells promoted myoblast proliferation by reducing the expression of myostatin, a critical inhibitor of skeletal myogenesis. In addition, the miR-27a was confirmed to target myostatin 3'UTR by a luciferase reporter analysis. Together, these results suggest that miR-27a promotes myoblast proliferation through targeting myostatin. (C) 2012 Elsevier Inc. All rights reserved.

引用

收藏

页码：265 / 269

页数：5

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