Identification of respiratory viruses with a novel point-of-care multianalyte antigen detection test in children with acute respiratory tract infection

Background: Rapid etiological diagnosis of a respiratory virus infection may have impact on antiviral and antibiotic therapy, patient cohorting, and prediction of the clinical course. Most point-of-care tests for detection of respiratory viruses have limitations in diagnostic performance and clinical usability. A novel, multianalyte point-of-care antigen detection test system (mariPOC (R); ArcDia International Oy Ltd., Turku, Finland) detects eight respiratory viruses (influenza A and B viruses, respiratory syncytial virus (RSV), adenovirus, human metapneumovirus, and parainfluenza type 1, 2, and 3 viruses) from a single nasopharyngeal swab specimen by a fully automated, random-access immunoassay method. Objectives: To evaluate mariPOC (R) point-of-care test system in comparison with reverse transcription polymerase chain reaction (RT-PCR) in a pediatric emergency department setting. Study design: Prospectively collected samples from 158 children (mean age, 1.8 years) with respiratory symptoms and/or fever were analyzed both by mariPOC (R) and by multiplex RT-PCR. Results: The sensitivities and specificities (95% confidence intervals) of the mariPOC (R) test were for influenza A (n = 7), 71% (38-100) and 100%; influenza B (n = 22), 86% (72-100) and 98% (95-100); RSV (n = 35), 89% (78-99) and 100%; adenovirus (n = 12), 25% (1-50) and 97% (95-99); and for human metapneumovirus (n = 8), 50% (15-85) and 100%, respectively. Parainfluenzaviruses were detected only in five patients. Conclusions: This novel point-of-care test system is a rapid, practical, and specific method for simultaneous detection of eight respiratory viruses. Compared with RT-PCR, its sensitivity is moderately high for detection of RSV and influenza viruses, and low for adenovirus. (c) 2013 Elsevier B.V. All rights reserved.