Characterization of selenium species in biological extracts by enhanced ion-pair liquid chromatography with inductively coupled plasma-mass spectrometry and by referenced electrospray ionization-mass spectrometry

被引:67
作者
Kotrebai, M
Bird, SM
Tyson, JF
Block, E
Uden, PC
机构
[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA
[2] SUNY Albany, Dept Chem, Albany, NY 12222 USA
基金
美国国家卫生研究院;
关键词
selenoamino acids; speciation; high performance liquid chromatography; inductively coupled plasma-mass spectrometry; electrospray ionization-mass spectrometry;
D O I
10.1016/S0584-8547(99)00086-5
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Selenium is an essential nutrient for humans; selenium compounds catalyze intermediate metabolism reactions and inhibit the toxic effects of heavy metals such as arsenic, cadmium and mercury. Some extracts of selenium-enriched biological materials show cancer preventive effects, tentatively attributable to the biological functions of selenoamino acids. An improved ion pair chromatographic method with methodological enhancements for the separation, qualitative and quantitative determination of non-volatile selenium compounds extracted from different samples has been developed using ICP-MS as an element-selective detector. Separation power early in the chromatogram was increased to baseline separation in the standard mixture as a result of decreasing spray chamber size from 97 to 14 mi, and increasing trifluoracetic acid (TFA) concentration in the mobile phase from 0.1 to 0.6%. The former pH was restored by the addition of ammonia to the mobile phase, which also served to increase the column recovery of inorganic anions. Calibration curves for different selenoamino acids showed statistically different behavior. Biological sample extracts were characterized using HPLC-ICP-MS. Mass spectral behavior of selenoamino acids, using electrospray and ion trap technology with direct infusion and liquid chromatographic sample introduction, is also reported. (C) 1999 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:1573 / 1591
页数:19
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