Expression and clinical significance of CacyBP/SIP in pancreatic cancer

被引：35

作者：

Chen, Xiong ^{[1
,2
]}

Han, Guohong ^{[1
,2
]}

Zhai, Huihong ^{[1
,2
]}

Zhang, Faming ^{[1
,2
]}

Wang, Jinbo ^{[1
,2
]}

Li, Xiaohua ^{[1
,2
]}

Huang, Sheng ^{[3
]}

Wang, Xin ^{[1
,2
]}

Fan, Daiming ^{[1
,2
]}

机构：

[1] Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China

[2] Fourth Mil Med Univ, Inst Digest Dis, Xian 710032, Shaanxi, Peoples R China

[3] Fourth Mil Med Univ, Xijing Hosp, Dept Hepatobiliary Surg, Xian 710032, Shaanxi, Peoples R China

来源：

PANCREATOLOGY | 2008年 / 8卷 / 4-5期

基金：

中国国家自然科学基金;

关键词：

CacyBP/SIP; carcinogenesis; pancreatic cancer;

D O I：

10.1159/000151774

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Aims: Calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP), a component of the ubiquitin-mediated proteolysis, could bind SKP1-CUL1-F box protein complex and participate in beta-catenin degradation, which was found to be related to the malignant phenotypes of gastric cancer and renal cancer. However, the role of CacyBP/SIP in pancreatic cancer progression still remains unclear. Therefore, the aim of the present study was to investigate the expression and clinical significance of CacyBP/SIP in pancreatic cancer. Methods: Immunohistochemistry was carried out on paraffin-embedded sections of pancreatic cancer and normal pancreatic tissues. In addition, Western blot and semiquantitative RT-PCR were carried out to analyze mRNA and protein expression of CacyBP/SIP in 8 pairs of freshly resected pancreatic cancer and their adjacent nontumorous tissue. Results: CacyBP/SIP expression was significantly increased in pancreatic cancer tissue (28/68 or 41.2%) and correlated with differentiation degree, higher TNM ( tumor, node, metastasis) stage and distance metastasis. Also, mRNA and protein expression of CacyBP/SIP were found to be at higher levels in almost all cancer tissues compared to adjacent tissues. Conclusions: CacyBP/SIP protein might play an important role in the process of pancreatic carcinogenesis and high-level CacyBP/SIP expression might be related to the malignant potential of pancreatic cancer. Copyright (C) 2008 S. Karger AG, Basel and IAP.

引用

页码：470 / 477

页数：8

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