Selection of Smart Small-Molecule Ligands: The Proof of Principle

被引：63

作者：

Drabovich, Andrei P. ^{[1
]}

Berezovski, Maxim V. ^{[1
]}

Musheev, Michael U. ^{[1
]}

Krylov, Sergey N. ^{[1
]}

机构：

[1] York Univ, Dept Chem, Toronto, ON M3J 1P3, Canada

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 01期

关键词：

NONEQUILIBRIUM CAPILLARY-ELECTROPHORESIS; EQUILIBRIUM MIXTURES; PROTEIN; DISCOVERY; APTAMERS;

D O I：

10.1021/ac8023813

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of drugs and diagnostics with desirable characteristics requires smart small-molecule ligands-ligands with predefined binding parameters of interaction with the target. Here, we propose a general approach for selection of such ligands from highly diverse combinatorial libraries of small molecules by methods of kinetic capillary electrophoresis (KCE). We deduct three fundamental requirements for the combinatorial library to suit the KCE-based selection of smart ligands and suggest a universal design of the library for selecting smart small-molecule ligands: every small molecule in the library is tagged with DNA that encodes the structure of the molecule. Finally, we use several DNA-tagged small molecules, which represent a hypothetical library, to prove experimentally selection of smart small-molecule ligands by the proposed approach.

引用

页码：490 / 494

页数：5

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