Applications of LC/MS in structure identifications of small molecules and proteins in drug discovery

被引：49

作者：

Chen, Guodong ^{[1
]}

Pramanik, Birendra N. ^{[1
]}

Liu, Yan-Hui ^{[1
]}

Mirza, Urooj A. ^{[1
]}

机构：

[1] Schering Plough Res Inst, Kenilworth, NJ 07033 USA

来源：

JOURNAL OF MASS SPECTROMETRY | 2007年 / 42卷 / 03期

关键词：

LC/MS; LC/MS/MS; APCI-MS; impurity; metabolite; therapeutic protein;

D O I：

10.1002/jms.1184

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

With advancements in ionization methods and instrumentation, liquid chromatography/mass spectrometry (LC/MS) has become a powerful technology for the characterization of small molecules and proteins. This article will illustrate the role of LC/MS analysis in drug discovery process. Examples will be given on high-throughput analysis, structural analysis of trace level impurities in drug substances, identification of metabolites, and characterization of therapeutic protein products for process improvement. Some unique MS techniques will also be discussed to demonstrate their effectiveness in facilitating structural identifications. Copyright (c) 2007 John Wiley & Sons, Ltd.

引用

收藏

页码：279 / 287

页数：9

相关论文

共 71 条

[1] The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins [J].

Adachi, Jun ;

Kumar, Chanchal ;

Zhang, Yanling ;

Olsen, Jesper V. ;

Mann, Matthias .

GENOME BIOLOGY, 2006, 7 (09)

[2] Direct high-resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry [J].

Bereman, Michael S. ;

Nyadong, Leonard ;

Fernandez, Facundo M. ;

Muddiman, David C. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (22) :3409-3411

[3] Proteomics by FTICR mass spectrometry: Top down and bottom up [J].

Bogdanov, B ;

Smith, RD .

MASS SPECTROMETRY REVIEWS, 2005, 24 (02) :168-200

[4] Mass spectrometry: Bottom-up or top-down? [J].

Chait, Brian T. .

SCIENCE, 2006, 314 (5796) :65-66

[5] Imaging mass spectrometry: Principles and potentials [J].

Chaurand, P ;

Schwartz, SA ;

Reyzer, ML ;

Caprioli, RA .

TOXICOLOGIC PATHOLOGY, 2005, 33 (01) :92-101

[6]

Chen G., 2007, HPLC PHARM SCI, P281, DOI [10.1002/9780470087954.ch7, DOI 10.1002/9780470087954.CH7]

[7] Combining desorption electrospray ionization mass spectrometry and nuclear magnetic resonance for differential metabolomics without sample preparation [J].

Chen, Huanwen ;

Pan, Zhengzheng ;

Talaty, Nari ;

Raftery, Daniel ;

Cooks, R. Graham .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (10) :1577-1584

[8] Desorption electrospray ionization mass spectrometry for high-throughput analysis of pharmaceutical samples in the ambient environment [J].

Chen, HW ;

Talaty, NN ;

Takáts, Z ;

Cooks, RG .

ANALYTICAL CHEMISTRY, 2005, 77 (21) :6915-6927

[9] Versatile new ion source for the analysis of materials in open air under ambient conditions [J].

Cody, RB ;

Laramée, JA ;

Durst, HD .

ANALYTICAL CHEMISTRY, 2005, 77 (08) :2297-2302

[10] Ambient mass spectrometry [J].

Cooks, RG ;

Ouyang, Z ;

Takats, Z ;

Wiseman, JM .

SCIENCE, 2006, 311 (5767) :1566-1570

← 1 2 3 4 5 6 7 8 →