A new technique for visualizing proanthocyanidins by light microscopy

被引：10

作者：

Brillouet, J-M ^{[1
]}

Escoute, J. ^{[2
]}

机构：

[1] Univ Montpellier 1, UMR OEnol Sci, Natl Inst Agron Res, INRA UMI, Montpellier, France

[2] CIRAD, UMR Plant Breeding & Dev, Montpellier, France

来源：

BIOTECHNIC & HISTOCHEMISTRY | 2012年 / 87卷 / 03期

关键词：

artifacts; caffeine; condensed tannins; ethanol; exogenous protein; light microscopy; localization; proanthocyanidins; VITIS-VINIFERA L; CV CABERNET FRANC; GRAPE BERRIES; CELL-WALLS; LOCALIZATION; HISTOCHEMISTRY; FLAVAN-3-OLS; FIXATION;

D O I：

10.3109/10520295.2011.603703

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a new technique for visualizing proanthocyanidin-containing elements in plant tissues. Our innovation is the fixation of condensed tannins with an exogenous protein prior to alcohol dehydration. In this way, tannins do not undergo partial solubilization during the dehydration sequence and appear as sharply contoured globules of various diameters.

引用

页码：195 / 200

页数：6

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