Association between High miR-211 microRNA Expression and the Poor Prognosis of Oral Carcinoma

被引:127
作者
Chang, K.-W.
Liu, C. J.
Chu, T.-H.
Cheng, H.-W.
Hung, P. S.
Hu, W. Y.
Lin, S. C.
机构
[1] Natl Yang Ming Univ, Inst Oral Biol, Sch Dent, Taipei, Taiwan
[2] Taipei MacKay Mem Hosp, Dept Oral & Maxillofacial Surg, Taipei, Taiwan
[3] Nursing & Mngt Coll, Taipei, Taiwan
[4] Biosettia, San Diego, CA USA
关键词
lentivirus; microRNA; miR-211; oral carcinoma;
D O I
10.1177/154405910808701116
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
MicroRNAs (miRNAs) are non-coding RNAs that play roles in gene silencing and may be involved in tumorigenesis. miR-211 was mapped to chromosome 15q13, a locus frequently altered in cancers. The role of miR-211in carcinogenesis has not been clearly defined, however. This study investigated the pathogenetic implications of miR-211 in oral carcinogenesis. An association was found between higher miR-211 expression and the most advanced nodal metastasis, vascular invasion, and poor prognosis of oral carcinoma. The function of enforced miR-211 expression in oral carcinoma cells was confirmed by the repression of LacZ in a reporter plasmid via miR-211 targeting. Enforced miR-211 expression significantly increased the proliferation, migration, and anchorage-independent colony formation of oral carcinoma cells, while it enhanced the tumorigenicity of only SAS high-grade oral carcinoma cells, but not OECM-1 non-tumorigenic cells. The findings suggest that high miR-211 expression may be associated with the progression of oral carcinoma and poor patient outcomes. Abbreviations: Ct, threshold cycle; EF1 alpha, elongation factor 1 alpha HNSCC, head and neck squamous cell carcinoma; NHOK, normal human oral keratinocyte; OPL, oral precancerous lesion; OSCC, oral squamous cell carcinoma; Q-RT-PCR, quantitative reverse-transcriptase polymerase chain-reaction.
引用
收藏
页码:1063 / 1068
页数:6
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