The diversity of intestinal microbiota of Mongolians living in Inner Mongolia, China

被引：30

作者：

Zhang, J. ^{[1
]}

Zheng, Y. ^{[1
]}

Guo, Z. ^{[1
]}

Qiao, J. ^{[1
]}

Gesudu, Q. ^{[1
]}

Sun, Z. ^{[1
]}

Huo, D. ^{[1
]}

Huang, W. ^{[1
]}

Hou, Q. ^{[1
]}

Kwok, L. ^{[1
]}

Zhang, H. ^{[1
]}

机构：

[1] Inner Mongolia Agr Univ, Dept Food Sci & Engn, Educ Minist PR China, Key Lab Dairy Biotechnol & Bioengn, Hohhot 010018, Peoples R China

来源：

BENEFICIAL MICROBES | 2013年 / 4卷 / 04期

基金：

中国国家自然科学基金;

关键词：

intestinal microbiota; Mongolia; DGGE; qPCR; 16S RIBOSOMAL-RNA; GUT MICROBIOTA; BACTERIA; IDENTIFICATION; LACTOBACILLUS; SUCCINATE;

D O I：

10.3920/BM2013.0028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

The Mongolian nationality has developed their unique lifestyle and dietary habit for thousands of years. However, by now, little research has been focused on Mongolian gut microbiota and how it is related to different dietary habits. In this study, denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction (qPCR) methods were applied to reveal the diversity of predominant gut bacteria of 48 healthy Mongolians recruited from Hohhot city and the Xilin Gol pasturing area in Inner Mongolia. Compared to similar studies of other nationalities, results from the present study have confirmed that the composition of Mongolian gut microbiota is highly similar at the phylum level (Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria) but variable at the genus level. Especially, the numbers of Phascolarctobacterium, Lactobacillus and Bifidobacterium are rather high. DGGE profiles of Lactobacillus and Bifidobacterium revealed that Lactobacillus casei, Bifidobacterium longum and Bifidobacterium animalis subsp. lactis were predominant in the gut of the Mongolian subjects studied. On the contrary, Lactobacillus helveticus was detected in every pasturing area Mongolian, but not in any of the Hohhot city Mongolians. qPCR results revealed that the numbers of Lactobacillus and Bifidobacterium of Xilin Gol Mongolians were significantly higher (P<0.05) than that of Hohhot Mongolians, whereas the numbers of Enterobacterium were significantly lower (P<0.05). In addition, by partial least squares discriminate analysis and cluster analysis of data generated from DGGE and qPCR experiments, a striking difference in the composition of intestinal microbiota of Mongolians living in Hohhot city and the Xilin Gol pasturing area has been found. This study clearly shows that diet affects the microbiota composition of Mongolians living in different circumstances, i.e. urban versus rural.

引用

页码：319 / 328

页数：10

共 30 条

[1]

Comparative Analysis of Human Gut Microbiota by Barcoded Pyrosequencing [J].