Adenoviral E1A protein nuclear import is preferentially mediated by importin α3 in vitro

被引：37

作者：

Köhler, M

Görlich, D

Hartmann, E

Franke, J

机构：

[1] Humboldt Univ, Max Delbrueck Ctr, HELIOS Clin, Franz Volhard Clin, D-13122 Berlin, Germany

[2] Heidelberg Univ, Ctr Mol Biol, D-6900 Heidelberg, Germany

[3] Med Univ Lubeck, Inst Biol, D-23538 Lubeck, Germany

来源：

VIROLOGY | 2001年 / 289卷 / 02期

关键词：

D O I：

10.1006/viro.2001.1151

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Nuclear import of some viral proteins depends on importin alpha proteins. However, no preferences of distinct alpha -importins for any viral protein import have been demonstrated. We used in vitro import assays and observed that all ubiquitously expressed human importin a isoforms mediate nuclear translocation of adenoviral Elk Competition with nucleoplasmin suggests that importin alpha3 is the most efficient import mediator of E1A. (C) 2001 Academic Press.

引用

收藏

页码：186 / 191

页数：6

相关论文

共 22 条

[1] Hepatitis delta antigen mediates the nuclear import of hepatitis delta virus RNA [J].

Chou, HC ;

Hsieh, TY ;

Sheu, GT ;

Lai, MMC .

JOURNAL OF VIROLOGY, 1998, 72 (05) :3684-3690

[2] Mediators of nuclear protein import target karyophilic proteins to pore complexes of cytoplasmic annulate lamellae [J].

Cordes, VC ;

Rackwitz, HR ;

Reidenbach, S .

EXPERIMENTAL CELL RESEARCH, 1997, 237 (02) :419-433

[3] Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway [J].

Elton, D ;

Simpson-Holley, M ;

Archer, K ;

Medcalf, L ;

Hallam, R ;

McCauley, J ;

Digard, P .

JOURNAL OF VIROLOGY, 2001, 75 (01) :408-419

[4]

Franke J, 2001, J CELL SCI, V114, P2641

[5] Transport between the cell nucleus and the cytoplasm [J].

Görlich, D ;

Kutay, U .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1999, 15 :607-660

[6]

Jans DA, 2000, BIOESSAYS, V22, P532, DOI 10.1002/(SICI)1521-1878(200006)22:6<532::AID-BIES6>3.0.CO

[7]

2-O

[8] Three distinct classes of the α-subunit of the nuclear pore-targeting complex (importin-α) are differentially expressed in adult mouse tissues [J].

Kamei, Y ;

Yuba, S ;

Nakayama, T ;

Yoneda, Y .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1999, 47 (03) :363-372

[9]

Köhler M, 1999, MOL CELL BIOL, V19, P7782

[10] Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family [J].

Kohler, M ;

Ansieau, S ;

Prehn, S ;

Leutz, A ;

Haller, H ;

Hartmann, E .

FEBS LETTERS, 1997, 417 (01) :104-108