Functional and comparative bioinformatic analysis of expressed genes from wheat spikes infected with Fusarium graminearum

被引:85
作者
Kruger, WM
Pritsch, C
Chao, SM
Muehlbauer, GJ [1 ]
机构
[1] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA
[2] Univ Calif Davis, Genet Resources Conservat Program, Davis, CA 95616 USA
关键词
disease resistance; genomics;
D O I
10.1094/MPMI.2002.15.5.445
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fusarium head blight, caused by the fungus Fusarium graminearum, is a major disease on wheat (Triticum aestivum L.). Expressed sequence tags (ESTs) were used to identify genes expressed during the wheat-F graminearum interaction. We generated 4,838 ESTs from a cDNA library prepared from spikes of the partially resistant cultivar Sumai 3 infected with E graminearum. These ESTs were composed of 2,831 singlet (single-copy transcripts) and 715 contigs (multiple-copy transcripts) for a total of 3,546 non-redundant sequences. Four sets of nonredundant sequences were identified. One set contains numerous, common biotic and abiotic stress-related genes. Many of these stress-related genes were represented by multiple ESTs, indicating that they are abundantly expressed. A second set comprised 16 nonredundant sequences from E graminearum that may be required for pathogenicity. A subset of these fungal genes encodes proteins associated with plant cell wall degradation. A third set of 326 nonredundant sequences had no DNA or amino acid sequence similarity to almost 1 million plant and over 7 million animal sequences in dbEST (as of 22 June 2001). Thus, these 326 nonredundant sequences have only been found in our E graminearum-infected 'Sumai 31 cDNA library. A fourth set of 29 nonredundant sequences was found in our E graminearum-infected wheat and another plant-pathogen interaction cDNA library. Some of these sequences encode proteins that may act in establishing various plant-fungal interactions.
引用
收藏
页码:445 / 455
页数:11
相关论文
共 76 条
  • [1] BETA-GALACTOSIDASE AND ITS SIGNIFICANCE IN RIPENING MANGO FRUIT
    ALI, ZM
    ARMUGAM, S
    LAZAN, H
    [J]. PHYTOCHEMISTRY, 1995, 38 (05) : 1109 - 1114
  • [2] Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P229, DOI DOI 10.1007/BF02672073
  • [3] The significance of digital gene expression profiles
    Audic, S
    Claverie, JM
    [J]. GENOME RESEARCH, 1997, 7 (10): : 986 - 995
  • [4] BAI GH, 1994, PLANT DIS, V78, P760, DOI 10.1094/PD-78-0760
  • [5] Bent AF, 1996, PLANT CELL, V8, P1757, DOI 10.1105/tpc.8.10.1757
  • [6] BOWLES DJ, 1990, ANNU REV BIOCHEM, V59, P873, DOI 10.1146/annurev.bi.59.070190.004301
  • [7] BUSHNELL WR, 2000, NATL FUS HEAD BLIGHT, P129
  • [8] Virulence-dependent induction of Hsp70/Hsc70 in tomato by Ralstonia solanacearum
    Byth, HA
    Kuun, KG
    Bornman, L
    [J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2001, 39 (7-8) : 697 - 705
  • [9] Characterization of SNP1, a cell wall-degrading trypsin, produced during infection by Stagonospora nodorum
    Carlile, AJ
    Bindschedler, LV
    Bailey, AM
    Bowyer, P
    Clarkson, JM
    Cooper, RM
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 2000, 13 (05) : 538 - 550
  • [10] STRUCTURAL MODELS OF PRIMARY-CELL WALLS IN FLOWERING PLANTS - CONSISTENCY OF MOLECULAR-STRUCTURE WITH THE PHYSICAL-PROPERTIES OF THE WALLS DURING GROWTH
    CARPITA, NC
    GIBEAUT, DM
    [J]. PLANT JOURNAL, 1993, 3 (01) : 1 - 30