Functional and comparative bioinformatic analysis of expressed genes from wheat spikes infected with Fusarium graminearum

被引:85
作者
Kruger, WM
Pritsch, C
Chao, SM
Muehlbauer, GJ [1 ]
机构
[1] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA
[2] Univ Calif Davis, Genet Resources Conservat Program, Davis, CA 95616 USA
关键词
disease resistance; genomics;
D O I
10.1094/MPMI.2002.15.5.445
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fusarium head blight, caused by the fungus Fusarium graminearum, is a major disease on wheat (Triticum aestivum L.). Expressed sequence tags (ESTs) were used to identify genes expressed during the wheat-F graminearum interaction. We generated 4,838 ESTs from a cDNA library prepared from spikes of the partially resistant cultivar Sumai 3 infected with E graminearum. These ESTs were composed of 2,831 singlet (single-copy transcripts) and 715 contigs (multiple-copy transcripts) for a total of 3,546 non-redundant sequences. Four sets of nonredundant sequences were identified. One set contains numerous, common biotic and abiotic stress-related genes. Many of these stress-related genes were represented by multiple ESTs, indicating that they are abundantly expressed. A second set comprised 16 nonredundant sequences from E graminearum that may be required for pathogenicity. A subset of these fungal genes encodes proteins associated with plant cell wall degradation. A third set of 326 nonredundant sequences had no DNA or amino acid sequence similarity to almost 1 million plant and over 7 million animal sequences in dbEST (as of 22 June 2001). Thus, these 326 nonredundant sequences have only been found in our E graminearum-infected 'Sumai 31 cDNA library. A fourth set of 29 nonredundant sequences was found in our E graminearum-infected wheat and another plant-pathogen interaction cDNA library. Some of these sequences encode proteins that may act in establishing various plant-fungal interactions.
引用
收藏
页码:445 / 455
页数:11
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