High sensitivity detection of 16s rRNA using peptide nucleic acid probes and a surface plasmon resonance biosensor

被引：62

作者：

Joung, Hyou-Arm ^{[1
,2
]}

Lee, Nae-Rym ^{[1
,2
]}

Lee, Seok Ki ^{[1
]}

Ahn, Junhyoung ^{[1
]}

Shin, Yong Beom ^{[1
]}

Choi, Ho-Suk ^{[2
]}

Lee, Chang-Soo ^{[2
]}

Kim, Sanghyo ^{[3
]}

Kim, Min-Gon ^{[1
]}

机构：

[1] KRIBB, BioNanotechnol Res Ctr, Taejon 305806, South Korea

[2] Chungnam Natl Univ, Dept Chem Engn, Taejon 305764, South Korea

[3] Kyungwon Univ, Dept BioNanotechnol, Songnam 461701, Gyeonggi Do, South Korea

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 630卷 / 02期

关键词：

Peptide nucleic acid; Surface plasmon resonance; 16s rRNA; Cationic nanoparticle;

D O I：

10.1016/j.aca.2008.10.001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A signal enhancing method allowing highly sensitive detection of E. coli 16s rRNA was developed using peptide nucleic acid (PNA) as a capture probe and a surface plasmon resonance (SPR) sensor as a detector. 16s rRNA has been used as a genetic marker for identification of organisms, and can be analyzed directly without PCR amplification due to the relatively high number of copies. PNA has a neutral backbone structure, therefore hybridization with 16s rRNA results in the ionic condition being changed from neutral to negative. A cationic Au nanoparticle was synthesized and used for signal amplification by ionic interaction with 16s rRNA hybridized on the PNA probe-immobilized SPR sensor chip. This method resulted in a detection limit of E. coli rRNA of 58.2 +/- 1.37 pg mL(-1). Using this analytical method, Staphylococcus aureus was detected without purification of rRNA. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：168 / 173

页数：6

共 26 条

[1] Portable system for microbial sample preparation and oligonucleotide microarray analysis [J].