Detection of nucleic acids using enzyme-catalyzed template-guided deposition of polyaniline

被引:60
作者
Gao, Zhiqiang [1 ]
Rafea, Siti [1 ]
Lim, Lay Har [1 ]
机构
[1] Inst Microelect, Singapore 117685, Singapore
关键词
D O I
10.1002/adma.200601090
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An electrochemical procedure for the detection of nucleic acids is realized byutilizing polyaniline as a signal generator for the transduction of nucleic acid hybridization events (see figure). The unique combination of enzymatic amplification and template-guided deposition can be used in conjunction with other detection techniques, and the sensitivity of the biosensor increases with increasing time.
引用
收藏
页码:602 / +
页数:6
相关论文
共 29 条
[1]  
[Anonymous], 1992, RECOMBINANT DNA
[2]   Quantitative analysis of mRNA amplification by in vitro transcription [J].
Baugh, L. R. ;
Hill, A. A. ;
Brown, E. L. ;
Hunter, Craig P. .
NUCLEIC ACIDS RESEARCH, 2001, 29 (05)
[3]   Prime time for real-time PCR [J].
Bonetta, L .
NATURE METHODS, 2005, 2 (04) :305-311
[4]   Synthesis of conducting polyelectrolyte complexes of polyaniline and poly(2-acrylamido-3-methyl-1-propanesulfonic acid) catalyzed by pH-stable palm tree peroxidase [J].
Caramyshev, AV ;
Evtushenko, EG ;
Ivanov, VF ;
Barcel, AR ;
Roig, MG ;
Shnyrov, VL ;
van Huystee, RB ;
Kurochkin, IN ;
Vorobiev, AK ;
Sakharov, IY .
BIOMACROMOLECULES, 2005, 6 (03) :1360-1366
[5]   PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES [J].
EGHOLM, M ;
BUCHARDT, O ;
CHRISTENSEN, L ;
BEHRENS, C ;
FREIER, SM ;
DRIVER, DA ;
BERG, RH ;
KIM, SK ;
NORDEN, B ;
NIELSEN, PE .
NATURE, 1993, 365 (6446) :566-568
[6]  
F Allen J Bard L.R., 2001, Electrochemical Methods: Fundamentals and Applications
[7]   Detection of microRNAs using electrocatalytic nanoparticle tags [J].
Gao, ZQ ;
Yang, ZC .
ANALYTICAL CHEMISTRY, 2006, 78 (05) :1470-1477
[8]   SNP detection using peptide nucleic acid probes and conjugated polymers: Applications in neurodegenerative disease identification [J].
Gaylord, BS ;
Massie, MR ;
Feinstein, SC ;
Bazan, GC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (01) :34-39
[9]   DNA hybridization detection with water-soluble conjugated polymers and chromophore-labeled single-stranded DNA [J].
Gaylord, BS ;
Heeger, AJ ;
Bazan, GC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (04) :896-900
[10]   A formula for thermal stability (Tm) prediction of PNA/DNA duplexes [J].
Giesen, U ;
Kleider, W ;
Berding, C ;
Geiger, A ;
Orum, H ;
Nielsen, PE .
NUCLEIC ACIDS RESEARCH, 1998, 26 (21) :5004-5006