Synthesis of conducting polyelectrolyte complexes of polyaniline and poly(2-acrylamido-3-methyl-1-propanesulfonic acid) catalyzed by pH-stable palm tree peroxidase

被引:52
作者
Caramyshev, AV
Evtushenko, EG
Ivanov, VF
Barcel, AR
Roig, MG
Shnyrov, VL
van Huystee, RB
Kurochkin, IN
Vorobiev, AK
Sakharov, IY
机构
[1] Moscow MV Lomonosov State Univ, Fac Chem, Dept Chem Engn, Moscow 119992, Russia
[2] Russian Acad Sci, Inst Electrochem, Moscow 119071, Russia
[3] Univ Murcia, Dept Plant Physiol, E-30100 Murcia, Spain
[4] Univ Salamanca, Fac Quim, Dept Quim Fis, E-37008 Salamanca, Spain
[5] Univ Salamanca, Fac Biol, Dept Bioquim & Biol Mol, Salamanca 37008, Spain
[6] Univ Western Ontario, Dept Plant Sci, London, ON N6A 5B7, Canada
[7] GV Plekhanov Russian Econ Acad, Div Chem, Moscow 113054, Russia
关键词
D O I
10.1021/bm049370w
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Comparison of the stability of five plant peroxidases (horseradish, royal palm tree leaf, soybean, and cationic and anionic peanut peroxidases) was carried out under acidic conditions favorable for synthesis of polyelectrolyte complexes of polyaniline (PANI). It demonstrates that palm tree peroxidase has the highest stability. Using this peroxidase as a catalyst, the enzymatic synthesis of polyelectrolyte complexes of PANI and poly(2-acrylamido-3-methyl-1-propanesulfonic acid) (PAMPS) was developed. The template polymerization of aniline was carried out in aqueous buffer at pH 2.8. Varying the concentrations of aniline, PAMPS, and hydrogen peroxide as reagents, favorable conditions for production of PANI were determined. UV-vis-NIR absorption and EPR demonstrated that PAMPS and PANI formed the electroactive complex similar to PANI doped traditionally using low molecular weight sulfortic acids. The effect of pH on conformational variability of the complex was evaluated by UV-vis spectroscopy. Atomic force microscopy showed that a size of the particles of the PANI-PAMPS complexes varied between 10 and 25 nm, depending on a concentration of PANIPS in the complex. The dc conductivity of the complexes depends also on the content of PAMPS, the higher conductivity being for the complexes containing the lower content of the polymeric template.
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收藏
页码:1360 / 1366
页数:7
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