Isolation and characterization of compactin resistant mutants of an astaxanthin synthesizing green alga Haematococcus pluvialis

被引：21

作者：

Chumpolkulwong, N ^{[1
]}

Kakizono, T ^{[1
]}

Handa, T ^{[1
]}

Nishio, N ^{[1
]}

机构：

[1] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,HIGASHIHIROSHIMA 739,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 03期

关键词：

D O I：

10.1023/A:1018330329357

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Haematococcus pluvialis mutants resistant to compactin, an inhibitor for 3-hydroxy-3-methylglutaryl coenzyme A reductase that is a key regulatory enzyme in the isoprenoid biosynthesis, were screened for higher astaxanthin producers. At 2 mM compactin, the wild type failed to grow, while two isolated mutants showed fairly good growth. In compactin-free medium, these resistant mutants have accumulated 1.4-2.0 fold higher astaxanthin than the wild type did. Based upon the specific enzyme activity assay, the mutant enzyme was found slightly less sensitive to compactin.

引用

页码：299 / 302

页数：4

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