Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice

被引:280
作者
Verma, SC
Ladha, JK
Tripathi, AK [1 ]
机构
[1] Banaras Hindu Univ, Fac Sci, Sch Biotechnol, Varanasi 221005, Uttar Pradesh, India
[2] Int Rice Res Inst, Soil & Water Sci Div, Manila 1099, Philippines
关键词
BIOLOG; BOX-PCR; deepwater rice; endophytes; plant colonization; plant growth promotion;
D O I
10.1016/S0168-1656(01)00333-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A study of the diversity of endophytic bacteria present in seeds of a deepwater rice variety revealed the presence of seven types of BOX-PCR fingerprints. In order to evaluate the plant growth promoting potential the presence of nitrogenase, indole acetic acid production and mineral phosphate solubilization were estimated in the representative BOX-PCR types. The seven representatives of BOX-PCR types produced indole acetic acid, reduced acetylene and showed specific immunological cross-reaction with anti-dinitrogenase reductase antibody. Only four types showed mineral phosphate solubilizing ability. Comparison of cellulase and pectinase activities showed differences among different BOX-PCR types. PCR fingerprinting data showed that one strain isolated from the surface sterilized seeds as well as the aerial parts of the seedlings of rice variety showed low cellulase and pectinase but relatively high ARA. On the basis of 16S rDNA nucleotide sequence and BIOLOG system of bacterial identification, this strain was identified as Pantoea agglomerans. For studying the endophytic colonization this strain was genetically tagged with the reporter gene, gusA. Histochemical analysis of the seedling grown in hydroponics showed that the tagged strain colonized the root surface, root hairs, root cap, points of lateral root emergence, root cortex and the stelar region. Treatment of the roots with 2,4-D produced short thickened lateral roots which showed better colonization by P. agglomerans. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:127 / 141
页数:15
相关论文
共 57 条
[31]   BIOLOGICAL NITROGEN-FIXATION IN NON-LEGUMINOUS FIELD CROPS - RECENT ADVANCES [J].
KENNEDY, IR ;
TCHAN, YT .
PLANT AND SOIL, 1992, 141 (1-2) :93-118
[32]   THE RHIZOSPHERE MICROFLORA OF WHEAT AND BARLEY WITH SPECIAL REFERENCE TO GRAM-NEGATIVE BACTERIA [J].
KLEEBERGER, A ;
CASTORPH, H ;
KLINGMULLER, W .
ARCHIVES OF MICROBIOLOGY, 1983, 136 (04) :306-311
[33]   Introduction: Assessing opportunities for nitrogen fixation in rice - a frontier project [J].
Ladha, JK ;
deBruijn, FJ ;
Malik, KA .
PLANT AND SOIL, 1997, 194 (1-2) :1-10
[34]   ISOLATION AND IDENTIFICATION OF NITROGEN-FIXING ENTEROBACTER-CLOACAE AND KLEBSIELLA-PLANTICOLA ASSOCIATED WITH RICE PLANTS [J].
LADHA, JK ;
BARRAQUIO, WL ;
WATANABE, I .
CANADIAN JOURNAL OF MICROBIOLOGY, 1983, 29 (10) :1301-1308
[35]  
Ladha JK, 2000, QUEST NITROGEN FIXAT, P33
[36]  
LUDDEN PW, 1989, CURR TOP CELL REGUL, V30, P23
[37]   CELL-ASSOCIATED PECTINOLYTIC AND CELLULOLYTIC ENZYMES IN RHIZOBIUM-LEGUMINOSARUM BIOVAR TRIFOLII [J].
MATEOS, PF ;
JIMENEZZURDO, JI ;
CHEN, J ;
SQUARTINI, AS ;
HAACK, SK ;
MARTINEZMOLINA, E ;
HUBBELL, DH ;
DAZZO, FB .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :1816-1822
[38]   POPULATION-DYNAMICS OF ENDOPHYTIC BACTERIA IN FIELD-GROWN SWEET CORN AND COTTON [J].
MCINROY, JA ;
KLOEPPER, JW .
CANADIAN JOURNAL OF MICROBIOLOGY, 1995, 41 (10) :895-901
[39]   Identification and characterization of bacterial endophytes of rice [J].
Mukhopadhyay, K ;
Garrison, NK ;
Hinton, DM ;
Bacon, CW ;
Khush, GS ;
Peck, HD ;
Datta, N .
MYCOPATHOLOGIA, 1996, 134 (03) :151-159
[40]   THE RIBOSOMAL DATABASE PROJECT [J].
OLSEN, GJ ;
OVERBEEK, R ;
LARSEN, N ;
MARSH, TL ;
MCCAUGHEY, MJ ;
MACIUKENAS, MA ;
KUAN, WM ;
MACKE, TJ ;
XING, YQ ;
WOESE, CR .
NUCLEIC ACIDS RESEARCH, 1992, 20 :2199-2200