Spatial distribution of Na+ and K+ channels in spinal dorsal horn neurones:: Role of the soma, axon and dendrites in spike generation

Spinal dorsal horn neurones play an important role in processing sensory information received from primary afferent fibers. The application of the patch-clamp technique to thin slices of rat spinal cord has enabled the study of ionic channels in visually identified dorsal horn neurones. The small soma of these neurones isolated from the slice by means of a novel method of entire soma isolation' has become a convenient model for investigating the properties and distributions of ionic channels. The present review summarizes results of recent experiments studying different types of voltage-gated Na+ and K+ channels expressed in dorsal horn neurones. Uneven distribution of the channels between the soma, axon and dendrites appears to play a major role in determining the neuronal excitability. The contribution of the soma, axon and dendrites to generation and propagation of the action potentials in central neurones is discussed. (C) 1999 Elsevier Science Ltd. All rights reserved.