Epithelial cells infected with respiratory syncytial virus are resistant to the anti-inflammatory effects of hydrocortisone

被引:21
作者
Bonville, CA
Mehta, PA
Krilov, LR
Rosenberg, HF
Domachowske, JB
机构
[1] SUNY Upstate Med Univ, Dept Pediat, Div Infect Dis, Syracuse, NY 13210 USA
[2] Winthrop Univ Hosp, Dept Pediat, Mineola, NY 11501 USA
[3] NIAID, Host Def Lab, NIH, Bethesda, MD 20892 USA
关键词
paramyxovirus; hydrocortisone; interleukin-8; macrophage inflammatory; protein; 1-alpha;
D O I
10.1006/cimm.2001.1869
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In this work we continue our study of the biochemical responses of respiratory epithelial cells to infection with human paramyxovirus pathogens. In our earlier studies, we detected elevated concentrations of the proinflammatory chemokines MIP-1alpha and IL-8 in upper and lower respiratory tract secretions from patients infected with respiratory syncytial virus (RSV). Here we demonstrate the same trend for individuals infected with parainfluenza virus (PIV), with elevated concentrations of MIP-1a and IL-8 (means of 309 +/- 51 and 2280 +/- 440 pg/ml/mg protein, respectively) detected in nasal wash samples from 17 patients with culture-positive PIV. Similar to our findings with RSV, cells of the HEp-2 epithelial line and primary cultures of human bronchial epithelial cells respond to PIV infection with production and release of both MIP-1alpha and IL-8. Addition of the glucocorticoid anti-inflammatory agent hydrocortisone (200-1000 ng/ml) attenuated the production of MIP-1alpha and IL-8 in PIV-infected cells while having minimal to no effect on the production of these mediators from cells infected with RSV. Neither virus infection resulted in a change in the total cellular concentration of glucocorticoid receptors, nor did hydrocortisone exert any differential effect on viral replication. As repression of chemokine production by epithelial cells is likely to result in diminished recruitment of proinflammatory leukocytes, these results may explain in part why glucocorticoid therapy reduces the symptoms associated with acute PIV infection, but have little to no effect in the overall outcome in the case of RSV. (C) 2001 Elsevier Science (USA).
引用
收藏
页码:134 / 140
页数:7
相关论文
共 32 条
[31]  
TAL A, 1983, PEDIATRICS, V71, P13
[32]   Randomised double blind placebo controlled trial of prednisolone in children admitted to hospital with respiratory syncytial virus bronchiolitis [J].
van Woensel, JBM ;
Wolfs, TFW ;
vanAalderen, WMC ;
Brand, PLP ;
Kimpen, JLL .
THORAX, 1997, 52 (07) :634-637