Enzymatic reduction of alpha-keto acids leading to L-amino acids, D- or L-hydroxy acids

被引：82

作者：

Krix, G

Bommarius, AS

Drauz, K

Kottenhahn, M

Schwarm, M

Kula, MR

机构：

[1] UNIV DUSSELDORF,INST ENZYME TECHNOL,D-52404 JULICH,GERMANY

[2] DEGUSSA AG,ORGAN CHEM R & D,ICFE,D-63403 HANAU,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1997年 / 53卷 / 01期

关键词：

alpha-keto acids; reductive amination; enzymatic reduction; L-neopentylglycine; leucine dehydrogenase; phenylalanine dehydrogenase; hydroxyisocaproate dehydrogenase;

D O I：

10.1016/S0168-1656(96)01657-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The substrate range of leucine dehydrogenase and phenylalanine dehydrogenase isolated from different organisms was investigated using a range of hydrophobic alpha-keto acids. Several aliphatic L-amino acids with bulky side chains were synthesized by reductive amination, L-neopentylglycine was produced on 30 kg scale. Besides the reductive amination of the alpha-keto acids these compounds were also investigated as substrates for hydroxyisocaproate dehydrogenase from Lactobacillus confusus and Lactobacillus casei, respectively. (C) 1997 Elsevier Science B.V.

引用

页码：29 / 39

页数：11

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