Single-step purification of recombinant Bm86 protein produced in Pichia pastoris by salting-out and by acid precipitation of contaminants

被引：8

作者：

Boue, O ^{[1
]}

Sanchez, K ^{[1
]}

Tamayo, G ^{[1
]}

Hernandez, L ^{[1
]}

Reytor, E ^{[1
]}

Enriquez, A ^{[1
]}

机构：

[1] CTR GENET ENGN & BIOTECHNOL, GAVACTM PROD DIV, HAVANA 6, CUBA

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 08期

关键词：

D O I：

10.1023/A:1018438805811

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two single-step purification methods were used to isolate the recombinant protein, rBm86, produced in Pichia pastoris. Salting-out results in a compromise between final purity and recovery of rBm86. At 15, 25 and 35% of ammonium sulphate saturation (pH 7), rBm86 concentration in the supernatant phase was proportional to the initial amount of protein. Acid precipitation of contaminants resulted in 98% purity and 98% recovery of rBm86. High aggregation of rBm86, forming particles of 28 nm, changed the isoelectric point of monomers (5.5), considering only the aminoacid sequence, to 4.5 for particles.

引用

页码：561 / 565

页数：5

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