Ionizing Radiation Induces Stemness in Cancer Cells

被引：161

作者：

Ghisolfi, Laura ^{[1
]}

Keates, Andrew C. ^{[1
]}

Hu, Xingwang ^{[1
]}

Lee, Dong-ki ^{[2
]}

Li, Chiang J. ^{[1
]}

机构：

[1] Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Skip Ackerman Ctr Mol Therapeut,Div Gastroenterol, Boston, MA 02215 USA

[2] Sungkyunkwan Univ, Dept Chem, Global Res Lab RNAi Med, Suwon, South Korea

来源：

PLOS ONE | 2012年 / 7卷 / 08期

关键词：

TUMOR-INITIATING CELLS; INDUCED GENOMIC INSTABILITY; ACUTE MYELOID-LEUKEMIA; SIDE-POPULATION; SELF-RENEWAL; MULTIDRUG-RESISTANCE; MELANOMA-CELLS; DIFFERENTIATION; GROWTH; CARCINOGENESIS;

D O I：

10.1371/journal.pone.0043628

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The cancer stem cell (CSC) model posits the presence of a small number of CSCs in the heterogeneous cancer cell population that are ultimately responsible for tumor initiation, as well as cancer recurrence and metastasis. CSCs have been isolated from a variety of human cancers and are able to generate a hierarchical and heterogeneous cancer cell population. CSCs are also resistant to conventional chemo-and radio-therapies. Here we report that ionizing radiation can induce stem cell-like properties in heterogeneous cancer cells. Exposure of non-stem cancer cells to ionizing radiation enhanced spherogenesis, and this was accompanied by upregulation of the pluripotency genes Sox2 and Oct3/4. Knockdown of Sox2 or Oct3/4 inhibited radiation-induced spherogenesis and increased cellular sensitivity to radiation. These data demonstrate that ionizing radiation can activate stemness pathways in heterogeneous cancer cells, resulting in the enrichment of a CSC subpopulation with higher resistance to radiotherapy.

引用

页数：11

共 75 条

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