Heme oxygenase and NO-synthase in the human prostate - Relation to adrenergic, cholinergic and peptide-containing nerves

被引:66
作者
Hedlund, P
Ekstrom, P
Larsson, B
Alm, P
Andersson, KE
机构
[1] LUND UNIV,DEPT CLIN PHARMACOL,LUND,SWEDEN
[2] LUND UNIV,DEPT ZOOL,S-22362 LUND,SWEDEN
[3] LUND UNIV,DEPT PATHOL,LUND,SWEDEN
来源
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM | 1997年 / 63卷 / 03期
关键词
prostate; non-adrenergic; non-cholinergic nerves; carbon monoxide; nitric oxide;
D O I
10.1016/S0165-1838(96)00139-7
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In the human prostate, the distribution of heme oxygenase (HO-1 and HO-2)-, nitric oxide synthase (NOS)-, and tyrosine hydroxylase (TH)-immunoreactive (IR), acetylcholine-esterase (AChE)-positive, and some peptidergic nerve structures was investigated. Cell bodies and nerve fibers within coarse nerve trunks expressed HO-1-, HO-2-, NOS-, TH-, and vasoactive intestinal polypeptide (VIP)-immunoreactivities, and were AChE-positive, but, as revealed by confocal microscopy, HO- and NOS-immunonactivities were found in separate nerves. Along strains of smooth muscle, intraglandular septa, and around acini, HO-1-, NOS-, and VIP-IR nerves, and AChE-positive fibers were observed. Double immunostaining showed that NOS- and VIP-immunoreactivities were generally co-localized in varicose nerve terminals. Some TH-IR terminals had profiles that were similar, but not identical, to those of NOS-, HO-1-, or VIP-LR terminals. NPY-IR nerves were similarly distributed as VIP- and NOS-IR fibers, and were found in rich amounts. Calcitonin gene-related peptide (CCRP)-IR nerves were few compared to other nerve populations studied. NOS- and CGRP-IR terminals had similar profiles, but the immunoreactivities were not co-localized. Nitric oxide and electrical stimulation of nerves relaxed noradrenaline-contracted preparations of prostatic stroma. Inhibition of synthesis of nitric oxide abolished the electrically induced relaxations. VIP had small relaxant effects, whereas carbon monoxide was without effect on noradrenaline-contracted strips. The innervation pattern and the functional effects suggest that the L-arginine/nitric oxide pathway may have a role in the control of human prostatic smooth muscle activity and/or in secretory neurotransmission. A physiological role of carbon monoxide in the prostate remains to be established.
引用
收藏
页码:115 / 126
页数:12
相关论文
共 53 条
[1]   IMMUNOHISTOCHEMICAL LOCALIZATION OF PERIPHERAL NITRIC-OXIDE SYNTHASE-CONTAINING NERVES USING ANTIBODIES RAISED AGAINST SYNTHESIZED C-TERMINAL AND N-TERMINAL FRAGMENTS OF A CLONED ENZYME FROM RAT-BRAIN [J].
ALM, P ;
LARSSON, B ;
EKBLAD, E ;
SUNDLER, F ;
ANDERSSON, KE .
ACTA PHYSIOLOGICA SCANDINAVICA, 1993, 148 (04) :421-429
[2]  
ANDERSON KE, 1993, PHARMACOL REV, V45, P253
[3]   THE L-ARGININE NITRIC-OXIDE PATHWAY AND NONADRENERGIC, NONCHOLINERGIC RELAXATION OF THE LOWER URINARY-TRACT [J].
ANDERSSON, KE ;
PERSSON, K .
GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM, 1993, 24 (04) :833-839
[4]  
ANDERSSON KE, 1995, CURR OPIN UROL, V5, P167
[5]   FUNCTIONS OF ADRENERGIC AND CHOLINERGIC NERVES IN CANINE EFFECTORS OF SEMINAL EMISSION [J].
ARVER, S ;
SJOSTRAND, NO .
ACTA PHYSIOLOGICA SCANDINAVICA, 1982, 115 (01) :67-77
[6]   CHARACTERIZATION AND LOCALIZATION OF NITRIC-OXIDE SYNTHASE IN THE HUMAN PROSTATE [J].
BURNETT, AL ;
MAGUIRE, MP ;
CHAMNESS, SL ;
RICKER, DD ;
TAKEDA, M ;
LEPOR, H ;
CHANG, TSK .
UROLOGY, 1995, 45 (03) :435-439
[7]  
CAINE M, 1986, J UROLOGY, V136, P1
[8]   THE INNERVATION OF THE HUMAN PROSTATE-GLAND - THE CHANGES ASSOCIATED WITH BENIGN ENLARGEMENT [J].
CHAPPLE, CR ;
CROWE, R ;
GILPIN, SA ;
GOSLING, J ;
BURNSTOCK, G .
JOURNAL OF UROLOGY, 1991, 146 (06) :1637-1644
[9]   THE HUMAN PROSTATE-GLAND - A HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL STUDY OF NEUROPEPTIDES, SEROTONIN, DOPAMINE BETA-HYDROXYLASE AND ACETYLCHOLINESTERASE IN AUTONOMIC NERVES AND GANGLIA [J].
CROWE, R ;
CHAPPLE, CR ;
BURNSTOCK, G .
BRITISH JOURNAL OF UROLOGY, 1991, 68 (01) :53-61
[10]  
Dail WG, 1993, AUTONOMIC NERVOUS SY, P69