SPR imaging of photo-cross-linked small-molecule arrays on gold

被引：97

作者：

Kanoh, N

Kyo, M

Inamori, K

Ando, A

Asami, A

Nakao, A

Osada, H

机构：

[1] RIKEN, Discovery Res Inst, Antibiot Lab, Wako, Saitama 3510198, Japan

[2] RIKEN, Discovery Res Inst, Beam Applicat Team, Wako, Saitama 3510198, Japan

[3] TOYOBO Co Ltd, Biotechnol Frontier Project, Tsuruga, Fukui 9140047, Japan

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 07期

关键词：

D O I：

10.1021/ac051777j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor a were observed using this platform.

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页码：2226 / 2230

页数：5

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