Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection

被引：204

作者：

Schotter, J

Kamp, PB

Becker, A

Pühler, A

Reiss, G

Brückl, H

机构：

[1] Univ Bielefeld, Dept Phys, D-33615 Bielefeld, Germany

[2] Univ Bielefeld, Dept Biol, D-33615 Bielefeld, Germany

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 19卷 / 10期

关键词：

biosensor; DNA; fluorescence; magnetic markers; magnetoresistive sensors; microarray;

D O I：

10.1016/j.bios.2003.11.007

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We present a comparative analysis of a magnetoresistive biosensor to standard fluorescent DNA detection. The biosensor consists of giant magnetoresistive (GMR) type Cu/Ni80Fe20 multilayers in the second antiferromagnetic coupling maximum. Each of the 206 elements of the magnetoresistive biosensor is patterned into a spiral-shaped line that can cover the area of a typical DNA spot (70 mum diameter). The probe DNA is assembled on top of the sensor elements in different concentrations ranging from 16 pg/mul to 10 ng/mul. Complementary biotin-labeled analyte DNA is hybridized to the probe DNA at a concentration of 10 ng/mul. A number of different commercially available magnetic microspheres are investigated to determine the most appropriate markers. The experimental comparison shows that the relative sensitivity of the magnetoresistive biosensor is superior to the fluorescent detection at low probe DNA concentrations. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：1149 / 1156

页数：8

共 19 条

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