Prokaryotic community profiles at different operational stages of a Greek solar saltern

被引:41
作者
Tsiamis, George [1 ]
Katsaveli, Katerina [1 ]
Ntougias, Spyridon [2 ]
Kyrpides, Nikos [3 ]
Andersen, Gary [4 ]
Piceno, Yvette [4 ]
Bourtzis, Kostas [1 ]
机构
[1] Univ Ioannina, Dept Environm & Nat Resources Management, GR-45110 Ioannina, Greece
[2] Natl Agr Res Fdn, Inst Kalamata, Kalamata 24100, Greece
[3] Genome Biol Program, Joint Genome Inst, Dept Energy, Walnut Creek, CA 94598 USA
[4] Univ Calif Berkeley, Lawrence Berkeley Lab, Ctr Environm Biotechnol, Berkeley, CA 94720 USA
关键词
Halophiles; Prokaryotic diversity; Hypersaline; PhyloChip; MikroBioKosmos;
D O I
10.1016/j.resmic.2008.09.007
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A combination of culture-dependent and independent approaches was employed to identify the microbial community structure in a Greek solar saltem. A total of 219 and 132 isolates belonging, respectively, to Bacteria and Archaea, were recovered. All bacterial isolates were phylogenetically related to 43 members of Actinobacteria, Firmicutes and gamma-Proteobacteria. The archaeal isolates were placed within the Halobacteriaceae. At least four groups of isolates represented novel species among the Bacteria. High bacterial diversity, consisting of 417 subfamilies, was revealed using a high-density oligonucleotide microarray (PhyloChip). At the four stages of saltem operation analyzed, the archaeal community consisted of both Crenarchaeota and Euryarchaeota, except for the sediment where Crenarchaeota were not detected. The bacterial community in sediment consisted mainly of gamma-Proteobacteria and Actinobacteria, while, in hypersaline water, it was restricted to a few representatives of Bacteria. Members of alpha-Proteobacteria were the main constituents in saturated brine and crude salt, followed by gamma-Proteobacteria, Actinobacteria and Firmicutes. A large Bacteroidetes and Verrucomicrobia diversity was identified in saturated brine, while delta-Proteobacteria and Cloroflexi were abundant in crude salt. Significant changes in the microbial community structure were detected during a short time period, denoting a rapidly adaptive dynamic ecosystem and viable diversity. Prokaryotic members reported for the first time in solar salterns were identified. (C) 2008 Elsevier Masson SAS. All fights reserved.
引用
收藏
页码:609 / 627
页数:19
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