Quantification of extrapulmonary translocation of intratracheal-instilled particles in vivo in rats: Effect of lipopolysaccharide

被引:89
作者
Chen, Jianmin
Tan, Mingguang
Nemmar, Abdeffahim
Song, Weiming
Dong, Mo
Zhang, Guilin
Li, Yan
机构
[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Lab Key Nucl Anal Tech, Shanghai 201800, Peoples R China
[2] Sultan Qaboos Univ, Coll Med, Dept Physiol, Al Khoud, Oman
[3] Fudan Univ, Sch Publ Hlth, Shanghai 200032, Peoples R China
[4] Chinese Acad Sci, Grad Sch, Shanghai 201800, Peoples R China
基金
中国国家自然科学基金;
关键词
air pollution; ultrafine; radioiodinated; polystyrene particles;
D O I
10.1016/j.tox.2006.02.016
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Particulate air pollution is associated with respiratory and cardiovascular morbidity and mortality. However, important uncertainties remain in the quantification of extrapulmonary translocation of ultrafine particles into blood circulation. Therefore, the widely used radioiodinated technique was applied to radiolabel polystyrene particles with an average diameter of 56.4 and 202 nm, respectively. The extrapulmonary distribution of these particles (3.7 x 10(5) Bq/rat) was quantified at 0.5, 2, 24 and 120 h after intratracheal instillation in rats. Moreover, we have taken into account the possible involvement of pulmonary inflammation in this process. Rats which received a single intratracheal instillation of free I-125 or a single intravenous injection of labeled ultrafine particles served as control. The results indicated that the pulmonary deposition of radioactivity was almost unchanged for both sizes. Only small amounts of radioactivity (1.64-2.49%) were recovered in blood shortly after administration of both types of particle, in healthy rats. However, the extent of particle translocation into the blood of the ultrafine size following the pretreatment with lipopolysaccharides was significantly higher (from 1.96 +/- 0.67 to 4.73 +/- 0.31%) compared to larger particles (from 2.19 +/- 0.77 to 2.21 +/- 0.64%). In conclusion, our findings suggest that only a small fraction of intratracheal-instilled ultrafine particles can pass rapidly into systemic circulation, but this translocation is markedly increased following LPS pretreatment. Thus, pulmonary inflammation seems to play a major role in enhancing the extrapulmonary translocation of particles. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:195 / 201
页数:7
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