A rapid tissue culture assay for the detection of okadaic acid and related compounds in mussels

被引：26

作者：

Croci, L

Cozzi, L

Stacchini, A

DeMedici, D

Toti, L

机构：

[1] Reparto Igiene delle Tecnologie A., Istituto Superiore di Sanità, 00161, Roma

来源：

TOXICON | 1997年 / 35卷 / 02期

关键词：

D O I：

10.1016/S0041-0101(96)00124-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Okadaic acid, the main toxin of the diarrhoetic shellfish poisoning (DSP) group, shows high cytotoxicity to Buffalo green monkey (BGM) kidney cell cultures after different times according to concentration. A rapid method of detecting the presence of okadaic acid and related compounds in contaminated mussels by means of a cytotoxicity test is presented. The method is based on direct microscopic observation of toxin-induced morphological changes in BGM cell cultures. A high correlation (r = 0.950; P < 0.001) was found between the official mouse bioassay and the cytotoxicity test conducted on naturally contaminated samples of Mytilus galloprovincialis. (C) 1997 Elsevier Science Ltd.

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收藏

页码：223 / 230

页数：8

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