Evidence for Novel Tomato Seed Allergens: IgE-Reactive Legumin and Vicilin Proteins Identified by Multidimensional Protein Fractionation-Mass Spectrometry and in Silico Epitope Modeling

被引：32

作者：

Baessler, Olivia Y. ^{[1
]}

Weiss, Julia ^{[2
]}

Wienkoop, Stefanie ^{[2
]}

Lehmann, Karola ^{[3
]}

Scheler, Christian ^{[3
]}

Doelle, Sabine ^{[4
]}

Schwarz, Dietmar ^{[5
]}

Franken, Philipp ^{[5
]}

George, Eckhard ^{[5
]}

Worm, Margitta ^{[4
]}

Weckwerth, Wolfram ^{[1
,2
]}

机构：

[1] Max Planck Inst Mol Plant Physiol, D-14467 Potsdam, Germany

[2] Univ Potsdam, GoFORSYS, D-14467 Potsdam, Germany

[3] Proteome Factory AG, D-10117 Berlin, Germany

[4] Allergie Ctr Charite, Dept Dermatol & Allergy, D-10117 Berlin, Germany

[5] Leibniz Inst Vegetable & Ornamental Crops IGZ, D-14979 Grossbeeren, Germany

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 03期

关键词：

food allergy; tomato; legumin; vicilin; IgE; mass spectrometry; LC-MS/MS; immunoblot; QUANTITATIVE SHOTGUN PROTEOMICS; ANACARDIUM-OCCIDENTALE L; PLANT FOOD ALLERGENS; ARA H 1; CROSS-REACTIVITY; ARABIDOPSIS-THALIANA; MUTATIONAL ANALYSIS; BINDING EPITOPES; AMINO-ACID; LATEX;

D O I：

10.1021/pr800186d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins.

引用

页码：1111 / 1122

页数：12

共 45 条

[1] ALCALA JVJ, 2000, GENERATION EST UNPUB
[2] [Anonymous], 2001, REP JOINT FAO WHO EX
[3] Detection of clinical markers of sensitization to profilin in patients allergic to plant-derived foods
Asero, R
Mistrello, G
Roncarolo, D
Amato, S
Zanoni, D
Barocci, F
Caldironi, G
[J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2003, 112 (02) : 427 - 432
[4] Latex allergy can induce clinical reactions to specific foods
Beezhold, DH
Sussman, GL
Liss, GM
Chang, NS
[J]. CLINICAL AND EXPERIMENTAL ALLERGY, 1996, 26 (04) : 416 - 422
[5] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6] Breiteneder H, 2004, J ALLERGY CLIN IMMUN, V113, P821, DOI [10.1016/j.jaci.2004.01.079, 10.1016/j.jaci.2004.01.779]
[7] Biological master games: Using biologists' reasoning to guide algorithm development for integrated functional genomics
Breitling, R
Herzyk, P
[J]. OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY, 2005, 9 (03) : 225 - 232
[8] Mapping and mutational analysis of the IgE-binding epitopes on Ara h 1, a legume vicilin protein and a major allergen in peanut hypersensitivity
Burks, AW
Shin, D
Cockrell, G
Stanley, JS
Helm, RM
Bannon, GA
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 245 (02): : 334 - 339
[9] DEMARTINO M, 1988, ALLERGY, V43, P206
[10] Cross-reactions in the latex-fruit syndrome: A relevant role of chitinases but not of complex asparagine-linked glycans
Diaz-Perales, A
Collada, C
Blanco, C
Sanchez-Monge, R
Carrillo, T
Aragoncillo, C
Salcedo, G
[J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1999, 104 (03) : 681 - 687

← 1 2 3 4 5 →