Evidence for Novel Tomato Seed Allergens: IgE-Reactive Legumin and Vicilin Proteins Identified by Multidimensional Protein Fractionation-Mass Spectrometry and in Silico Epitope Modeling

被引：32

作者：

Baessler, Olivia Y. ^{[1
]}

Weiss, Julia ^{[2
]}

Wienkoop, Stefanie ^{[2
]}

Lehmann, Karola ^{[3
]}

Scheler, Christian ^{[3
]}

Doelle, Sabine ^{[4
]}

Schwarz, Dietmar ^{[5
]}

Franken, Philipp ^{[5
]}

George, Eckhard ^{[5
]}

Worm, Margitta ^{[4
]}

Weckwerth, Wolfram ^{[1
,2
]}

机构：

[1] Max Planck Inst Mol Plant Physiol, D-14467 Potsdam, Germany

[2] Univ Potsdam, GoFORSYS, D-14467 Potsdam, Germany

[3] Proteome Factory AG, D-10117 Berlin, Germany

[4] Allergie Ctr Charite, Dept Dermatol & Allergy, D-10117 Berlin, Germany

[5] Leibniz Inst Vegetable & Ornamental Crops IGZ, D-14979 Grossbeeren, Germany

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 03期

关键词：

food allergy; tomato; legumin; vicilin; IgE; mass spectrometry; LC-MS/MS; immunoblot; QUANTITATIVE SHOTGUN PROTEOMICS; ANACARDIUM-OCCIDENTALE L; PLANT FOOD ALLERGENS; ARA H 1; CROSS-REACTIVITY; ARABIDOPSIS-THALIANA; MUTATIONAL ANALYSIS; BINDING EPITOPES; AMINO-ACID; LATEX;

D O I：

10.1021/pr800186d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins.

引用

页码：1111 / 1122

页数：12

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