Evolution of Bacillus thuringiensis Cry toxins insecticidal activity

被引：215

作者：

Bravo, Alejandra ^{[1
]}

Gomez, Isabel ^{[1
]}

Porta, Helena ^{[1
]}

Ines Garcia-Gomez, Blanca ^{[1
]}

Rodriguez-Almazan, Claudia ^{[1
]}

Pardo, Liliana ^{[1
]}

Soberon, Mario ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62250, Morelos, Mexico

来源：

MICROBIAL BIOTECHNOLOGY | 2013年 / 6卷 / 01期

关键词：

MODIFIED BT TOXINS; DELTA-ENDOTOXIN; PHAGE DISPLAY; DOMAIN-II; CRYSTAL PROTEINS; FIELD-RESISTANCE; MANDUCA-SEXTA; TOXICITY; BINDING; SPECIFICITY;

D O I：

10.1111/j.1751-7915.2012.00342.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Insecticidal Cry proteins produced by Bacillus thuringiensis are use worldwide in transgenic crops for efficient pest control. Among the family of Cry toxins, the three domain Cry family is the better characterized regarding their natural evolution leading to a large number of Cry proteins with similar structure, mode of action but different insect specificity. Also, this group is the better characterized regarding the study of their mode of action and the molecular basis of insect specificity. In this review we discuss how Cry toxins have evolved insect specificity in nature and analyse several cases of improvement of Cry toxin action by genetic engineering, some of these examples are currently used in transgenic crops. We believe that the success in the improvement of insecticidal activity by genetic evolution of Cry toxins will depend on the knowledge of the rate-limiting steps of Cry toxicity in different insect pests, the mapping of the specificity binding regions in the Cry toxins, as well as the improvement of mutagenesis strategies and selection procedures.

引用

页码：17 / 26

页数：10

共 63 条

[61] Enhanced toxicity of Bacillus thuringiensis Cry3A δ-endotoxin in coleopterans by mutagenesis in a receptor binding loop [J].

Wu, SJ ;

Koller, CN ;

Miller, DL ;

Bauer, LS ;

Dean, DH .

FEBS LETTERS, 2000, 473 (02) :227-232

[62] N546 in β18-β19 loop is important for binding and toxicity of the Bacillus thuringiensis Cry1Ac toxin [J].

Xiang, Wang Fa ;

Qiu, Xia Li ;

Zhi, Ding Xue ;

Min, Zhao Xin ;

Yuan, Lv ;

Quan, Yu Zi .

JOURNAL OF INVERTEBRATE PATHOLOGY, 2009, 101 (02) :119-123

[63] A mechanism of cell death involving an adenylyl cyclase/PKA signaling pathway is induced by the Cry1Ab toxin of Bacillus thuringiensis [J].

Zhang, Xuebin ;

Candas, Mehmet ;

Griko, Natalya B. ;

Taussig, Ronald ;

Bulla, Lee A., Jr. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (26) :9897-9902

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