Nitric oxide regulates human sperm capacitation and protein-tyrosine phosphorylation in vitro

被引：90

作者：

Herrero, MB ^{[1
]}

de Lamirande, E ^{[1
]}

Gagnon, C ^{[1
]}

机构：

[1] McGill Univ, Urol Res Lab, Royal Victoria Hosp, Montreal, PQ H3A 1A1, Canada

来源：

BIOLOGY OF REPRODUCTION | 1999年 / 61卷 / 03期

关键词：

D O I：

10.1095/biolreprod61.3.575

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of the present study was to investigate whether the generation of nitric oxide by human spermatozoa is associated with human sperm capacitation and with the tyrosine phosphorylation of sperm proteins. Human spermatozoa were capacitated in the presence or absence of nitric oxide-releasing compounds or nitric oxide synthase inhibitors, and then the percentage of acrosome loss induced by human follicular fluid or by calcium ionophore was determined. The presence of the nitric oxide-releasing compounds primed spermatozoa to respond earlier to human follicular fluid whereas nitric oxide synthase inhibitors decreased the percentage of acrosome reaction. Moreover, nitric oxide modulated tyrosine phosphorylation of sperm proteins. A tight correlation between capacitation and tyrosine phosphorylation regulated by nitric oxide was observed. Results indicate that nitric oxide is involved in human sperm capacitation and emphasize the importance of oxidoreduction reactions in the fine control of sperm physiology.

引用

页码：575 / 581

页数：7

共 41 条

[1]

Aitken RJ, 1998, J CELL SCI, V111, P645

[2]

AITKEN RJ, 1993, J ANDROL, V14, P132

[3] Potentiation of nitric oxide synthase expression by superoxide in interleukin 1β-stimulated rat mesangial cells [J].

Beck, KF ;

Eberhardt, W ;

Walpen, S ;

Apel, M ;

Pfeilschifter, J .

FEBS LETTERS, 1998, 435 (01) :35-38

[4]

BIELFELD P, 1994, FERTIL STERIL, V62, P1255

[5] ISOLATION OF NITRIC-OXIDE SYNTHETASE, A CALMODULIN-REQUIRING ENZYME [J].

BREDT, DS ;

SNYDER, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) :682-685

[6]

CALVO L, 1989, FERTIL STERIL, V52, P1048

[7] CHEMICAL-COMPOSITION AND PROTEIN-SOURCE IN THE CAPACITATION MEDIUM SIGNIFICANTLY AFFECT THE ABILITY OF HUMAN SPERMATOZOA TO UNDERGO FOLLICULAR-FLUID INDUCED ACROSOME REACTION [J].

CALVO, L ;

DENNISONLAGOS, L ;

BANKS, SM ;

FUGGER, EF ;

SHERINS, RJ .

HUMAN REPRODUCTION, 1993, 8 (04) :575-580

[8]

CORSS NL, 1986, GAMETE RES, V15, P235

[9]

de Lamirande E, 1998, J ANDROL, V19, P215

[10] Capacitation as a regulatory event that primes spermatozoa for the acrosome reaction and fertilization [J].

deLamirande, E ;

Leclerc, P ;

Gagnon, C .

MOLECULAR HUMAN REPRODUCTION, 1997, 3 (03) :175-194

← 1 2 3 4 5 →