Nitric oxide regulates human sperm capacitation and protein-tyrosine phosphorylation in vitro

被引：90

作者：

Herrero, MB ^{[1
]}

de Lamirande, E ^{[1
]}

Gagnon, C ^{[1
]}

机构：

[1] McGill Univ, Urol Res Lab, Royal Victoria Hosp, Montreal, PQ H3A 1A1, Canada

来源：

BIOLOGY OF REPRODUCTION | 1999年 / 61卷 / 03期

关键词：

D O I：

10.1095/biolreprod61.3.575

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of the present study was to investigate whether the generation of nitric oxide by human spermatozoa is associated with human sperm capacitation and with the tyrosine phosphorylation of sperm proteins. Human spermatozoa were capacitated in the presence or absence of nitric oxide-releasing compounds or nitric oxide synthase inhibitors, and then the percentage of acrosome loss induced by human follicular fluid or by calcium ionophore was determined. The presence of the nitric oxide-releasing compounds primed spermatozoa to respond earlier to human follicular fluid whereas nitric oxide synthase inhibitors decreased the percentage of acrosome reaction. Moreover, nitric oxide modulated tyrosine phosphorylation of sperm proteins. A tight correlation between capacitation and tyrosine phosphorylation regulated by nitric oxide was observed. Results indicate that nitric oxide is involved in human sperm capacitation and emphasize the importance of oxidoreduction reactions in the fine control of sperm physiology.

引用

页码：575 / 581

页数：7

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