PTP1D is a positive regulator of the prolactin signal leading to beta-casein promoter activation

被引：126

作者：

Ali, S

Chen, ZJ

Lebrun, JJ

Vogel, W

Kharitonenkov, A

Kelly, PA

Ullrich, A

机构：

[1] MAX PLANCK INST BIOCHEM, DEPT MOLEC BIOL, D-82152 MARTINSRIED, GERMANY

[2] FAC MED NECKER ENFANTS MALAD, INSERM, U344, F-75730 PARIS, FRANCE

来源：

EMBO JOURNAL | 1996年 / 15卷 / 01期

关键词：

Jak2; prolactin; prolactin receptor; protein tyrosine phosphatase 1D; signal transduction;

D O I：

10.1002/j.1460-2075.1996.tb00341.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stimulation of the prolactin receptor (PRLR), a member of the cytokine/growth hormone receptor family, results in activation of the associated Jak2 tyrosine kinase and downstream signaling pathways. We report that PTP1D, a cytoplasmic protein tyrosine phosphatase containing two Src homology 2 (SH2) domains, physically associates with the PRLR-Jak2 complex and is tyrosine-phosphorylated upon stimulation with prolactin. The formation of the trimeric PRLR-Jak2-PTP1D complex is critical for transmission of a lactogenic signal, while PTP1D phosphorylation is necessary, but not sufficient. The dominant negative inhibitory effect of a phosphatase-deficient mutant on expression of a beta-casein promoter-controlled reporter gene is evidence for an essential role of fully functional PTP1D in the regulation of milk protein gene transcription.

引用

页码：135 / 142

页数：8