Functional Effects of let-7g Expression in Colon Cancer Metastasis

被引：11

作者：

Chang, Che-Mai ^{[1
,2
,3
]}

Wong, Henry Sung-Ching ^{[1
,2
]}

Huang, Chien-Yu ^{[4
,5
]}

Hsu, Wen-Li ^{[6
,7
,8
]}

Maio, Zhi-Feng ^{[9
]}

Chiu, Siou-Jin ^{[10
]}

Tsai, Yao-Ting ^{[10
]}

Chen, Ben-Kuen ^{[11
]}

Wan, Yu-Jui Yvonne ^{[12
]}

Wang, Jaw-Yuan ^{[1
,9
,13
,14
,15
]}

Chang, Wei-Chiao ^{[1
,2
,16
,17
]}

机构：

[1] Taipei Med Univ, Sch Pharm, Master Program Clin Pharmacogen & Pharmacoprote, Taipei 110, Taiwan

[2] Taipei Med Univ, Sch Pharm, Dept Clin Pharm, Taipei 110, Taiwan

[3] Taipei Med Univ, Coll Med Sci & Technol, PhD Med Biotechnol Program, Taipei 110, Taiwan

[4] Taipei Med Univ, Coll Med, Sch Med, Dept Surg, Taipei 110, Taiwan

[5] Taipei Med Univ, Shuang Ho Hosp, Dept Surg, Div Gen Surg, Taipei 235, Taiwan

[6] Waseda Univ, Res Org Nano & Life Innovat, Tokyo 1628480, Japan

[7] Natl Pingtung Univ Sci & Technol, Emerging Cpds Res Ctr, Dept Environm Sci & Engn, Coll Engn, Pingtung 91201, Taiwan

[8] I Shou Univ, E DA Hosp, Dept Med Res, Kaohsiung 84001, Taiwan

[9] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Surg, Div Colorectal Surg, Kaohsiung 807, Taiwan

[10] Kaohsiung Med Univ, Coll Med, Dept Med Genet, Kaohsiung 807, Taiwan

[11] Natl Cheng Kung Univ, Coll Med, Dept Pharmacol, Tainan 701, Taiwan

[12] Univ Calif Davis, Dept Med Pathol & Lab Med, Sacramento, CA 95817 USA

[13] Kaohsiung Med Univ, Coll Med, Fac Med, Dept Surg, Kaohsiung 807, Taiwan

[14] Kaohsiung Med Univ, Coll Med, Grad Inst Med, Kaohsiung 807, Taiwan

[15] Kaohsiung Med Univ, Coll Med, Grad Inst Clin Med, Kaohsiung 807, Taiwan

[16] Taipei Med Univ, Dept Pharm, Wanfang Hosp, Taipei 116, Taiwan

[17] Taipei Med Univ, Shuang Ho Hosp, Dept Med Res, New Taipei 235, Taiwan

来源：

CANCERS | 2019年 / 11卷 / 04期

关键词：

colorectal cancer; microRNA; let-7g; the cancer genome atlas (TCGA); store-operated calcium (SOC); COLORECTAL-CANCER; CELL-MIGRATION; GENE ACTIVATION; MICRORNA; INVASION; PATHWAY; STIM1; SUPPRESSION; PROGRESSION; HSA-LET-7G;

D O I：

10.3390/cancers11040489

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment.

引用

页数：18

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