Split-seed: a new tool for maize researchers

被引:29
作者
Al-Abed, D [1 ]
Rudrabhatla, S [1 ]
Talla, R [1 ]
Goldman, S [1 ]
机构
[1] Univ Toledo, Plant Sci Res Ctr, Toledo, OH 43606 USA
关键词
dicotyledons; transgenic; maize; monocotyledons; regeneration; split-seed;
D O I
10.1007/s00425-006-0237-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Until recently, immature embryos have been a choice tissue for manipulation in culture for regeneration and production of transgenic maize plants. The utility of this explant has been compromised by low output, genotype dependence and time-consuming incubation in tissue culture. We have developed a new explant, the split-seed, which addresses these limitations by formally treating each seed as though it were a "dicot". By splitting maize seed longitudinally, three different tissues: the scutellum, the coleoptilar-ring and the shoot apical meristems are simultaneously exposed. The cells of these tissues can be made competent to enhance the regeneration, given that the molecular networks resulting from exposure of the split-seed to hormones is likely to be different from whole seed and, in turn, affects the in vitro response. Using this explant, callus induction frequency exceeded 92% and the regeneration frequency was 76%. The mean number of shoots regenerated via callus was 11 shoots per callus clump and 28 shoots per explant at first sub-culture. All of the regenerated plants survived and were 95% fertile. The large numbers of fertile plants produced were regenerated in 6-8 weeks. Finally, the incidence of regenerated plants varies as a function of growth regulator profile.
引用
收藏
页码:1355 / 1360
页数:6
相关论文
共 18 条
[1]   IMPROVED TISSUE-CULTURE RESPONSE OF AN ELITE MAIZE INBRED THROUGH BACKCROSS BREEDING, AND IDENTIFICATION OF CHROMOSOMAL REGIONS IMPORTANT FOR REGENERATION BY RFLP ANALYSIS [J].
ARMSTRONG, CL ;
ROMEROSEVERSON, J ;
HODGES, TK .
THEORETICAL AND APPLIED GENETICS, 1992, 84 (5-6) :755-762
[2]   ESTABLISHMENT AND MAINTENANCE OF FRIABLE, EMBRYOGENIC MAIZE CALLUS AND THE INVOLVEMENT OF L-PROLINE [J].
ARMSTRONG, CL ;
GREEN, CE .
PLANTA, 1985, 164 (02) :207-214
[3]   REGENERATION IN CEREAL TISSUE-CULTURE - A REVIEW [J].
BHASKARAN, S ;
SMITH, RH .
CROP SCIENCE, 1990, 30 (06) :1328-1337
[4]  
CHANG WF, 1983, PLANT CELL REP, V2, P183
[5]   Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system [J].
Frame, BR ;
Shou, HX ;
Chikwamba, RK ;
Zhang, ZY ;
Xiang, CB ;
Fonger, TM ;
Pegg, SEK ;
Li, BC ;
Nettleton, DS ;
Pei, DQ ;
Wang, K .
PLANT PHYSIOLOGY, 2002, 129 (01) :13-22
[6]   Production of transgenic maize from bombarded type II callus: Effect of gold particle size and callus morphology on transformation efficiency [J].
Frame, BR ;
Zhang, HY ;
Cocciolone, SM ;
Sidorenko, LV ;
Dietrich, CR ;
Pegg, SE ;
Zhen, SF ;
Schnable, PS ;
Wang, K .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2000, 36 (01) :21-29
[7]   NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].
GAMBORG, OL ;
MILLER, RA ;
OJIMA, K .
EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+
[8]  
GORDONKAMM WJ, 1990, PLANT CELL, V2, P603, DOI 10.1105/tpc.2.7.603
[9]   PLANT REGENERATION FROM TISSUE-CULTURES OF MAIZE [J].
GREEN, CE ;
PHILLIPS, RL .
CROP SCIENCE, 1975, 15 (03) :417-421
[10]   High-frequency plant regeneration through callus initiation from mature embryos of maize (Zea Mays L.) [J].
Huang, XQ ;
Wei, ZM .
PLANT CELL REPORTS, 2004, 22 (11) :793-800