Independent recruitment of mediator and SAGA by the activator Met4

被引:33
作者
Leroy, C [1 ]
Cormier, L [1 ]
Kuras, L [1 ]
机构
[1] CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France
关键词
D O I
10.1128/MCB.26.8.3149-3163.2006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mediator is a key RNA polymerase II (Pol II) cofactor in the regulation of eukaryotic gene expression. It is believed to function as a coactivator linking gene-specific activators to the basal Pol H initiation machinery. In support of this model, we provide evidence that Mediator serves in vivo as a coactivator for the yeast activator Met4, which controls the gene network responsible for the biosynthesis of sulfur-containing amino acids and S-adenosylmethionine. In addition, we show that SAGA (Spt-Ada-Gcn5-acetyltransferase) is also recruited to Met4 target promoters, where it participates in the recruitment of Pol II by a mechanism involving histone acetylation. Interestingly, we find that SAGA is not required for Mediator recruitment by Met4 and vice versa. Our results provide a novel example of functional interplay between Mediator and coactivators involved in histone modification.
引用
收藏
页码:3149 / 3163
页数:15
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