PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

被引：145

作者：

Ebralidze, Alexander K. ^{[1
]}

Guibal, Florence C. ^{[1
]}

Steidl, Ulrich ^{[2
,3
]}

Zhang, Pu ^{[1
]}

Lee, Sanghoon ^{[1
]}

Bartholdy, Boris ^{[1
]}

Jorda, Meritxell Alberich ^{[1
]}

Petkova, Victoria ^{[1
]}

Rosenbauer, Frank ^{[4
]}

Huang, Gang ^{[1
]}

Dayaram, Tajhal ^{[1
]}

Klupp, Johanna ^{[1
]}

O'Brien, Karen B. ^{[1
]}

Will, Britta ^{[1
]}

Hoogenkamp, Maarten ^{[5
]}

Borden, Katherine L. B. ^{[6
,7
]}

Bonifer, Constanze ^{[5
]}

Tenen, Daniel G. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Harbin Inst Med, Harvard Stem Cell Inst, Boston, MA 02115 USA

[2] Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA

[3] Albert Einstein Coll Med, Albert Einstein Canc Ctr, Bronx, NY 10461 USA

[4] Max Delbruck Ctr Mol Med, D-13092 Berlin, Germany

[5] Univ Leeds, St Jamess Univ Hosp, Leeds Inst Mol Med, Leeds LS7 4JS, W Yorkshire, England

[6] Univ Montreal, Dept Pathol & Cell Biol, Montreal, PQ H3T 1J4, Canada

[7] Univ Montreal, Inst Res Immunol & Canc, Montreal, PQ H3T 1J4, Canada

来源：

GENES & DEVELOPMENT | 2008年 / 22卷 / 15期

关键词：

noncoding antisense RNA; upstream and intronic regulatory elements; coordinated expression of the target and regulator; translation stalling;

D O I：

10.1101/gad.1654808

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.

引用

页码：2085 / 2092

页数：8

共 35 条

[21] ATRA resolves the differentiation block in t(15;17) acute myeloid leukemia by restoring PU.1 expression
Mueller, BU
Pabst, T
Fos, J
Petkovic, V
Fey, MF
Asou, N
Buergi, U
Tenen, DG
[J]. BLOOD, 2006, 107 (08) : 3330 - 3338
[22] Potential autoregulation of transcription factor PU.1 by an upstream reaulatory element
Okuno, Y
Huang, G
Rosenbauer, F
Evans, EK
Radomska, HS
Iwasaki, H
Akashi, K
Moreau-Gachelin, F
Li, YL
Zhang, P
Göttgens, B
Tenen, DG
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (07) : 2832 - 2845
[23] Lymphoid cell growth and transformation are suppressed by a key regulatory element of the gene encoding PU.1
Rosenbauer, F
Owens, BM
Yu, L
Tumang, JR
Steidl, U
Kutok, JL
Clayton, LK
Wagner, K
Scheller, M
Iwasaki, H
Liu, CH
Hackanson, B
Akashi, K
Leutz, A
Rothstein, TL
Plass, C
Tenen, DG
[J]. NATURE GENETICS, 2006, 38 (01) : 27 - 37
[24] Acute myeloid leukemia induced by graded reduction of a lineage-specific transcription factor, PU.1
Rosenbauer, F
Wagner, K
Kutok, JL
Iwasaki, H
Le Beau, MM
Okuno, Y
Akashi, K
Fiering, S
Tenen, DG
[J]. NATURE GENETICS, 2004, 36 (06) : 624 - 630
[25] Elements of transcription factor network design for T-lineage specification
Rothenberg, EV
Anderson, MK
[J]. DEVELOPMENTAL BIOLOGY, 2002, 246 (01) : 29 - 44
[26] RNA-mediated response to heat shock in mammalian cells
Shamovsky, I
Ivannikov, M
Kandel, ES
Gershon, D
Nudler, E
[J]. NATURE, 2006, 440 (7083) : 556 - 560
[27] The non-coding Air RNA is required for silencing autosomal imprinted genes
Sleutels, F
Zwart, R
Barlow, DP
[J]. NATURE, 2002, 415 (6873) : 810 - 813
[28] Interchromosomal associations between alternatively expressed loci
Spilianakis, CG
Lalioti, MD
Town, T
Lee, GR
Flavell, RA
[J]. NATURE, 2005, 435 (7042) : 637 - 645
[29] A distal single nucleotide polymorphism alters long-range reguiavon of the PU-1 gene in acute myeloid leukemia
Steidl, Ulrich
Steidl, Christian
Ebralidze, Alexander
Chapuy, Bjoen
Han, Hye-Jung
Will, Britta
Rosenbauer, Frank
Becker, Annegret
Wagner, Katharina
Koschmieder, Steffen
Kobayashi, Susumu
Costa, Daniel B.
Schuiz, Thomas
O'Brien, Karen B.
Verhaak, Roel G. W.
Dawel, Ruud
Haase, Detlef
Truemper, Lorenz
Krauter, Juergen
Kohwi-ShigematSU, Terumi
Griesinger, Frank
Tenen, Daniel G.
[J]. JOURNAL OF CLINICAL INVESTIGATION, 2007, 117 (09) : 2611 - 2620
[30] Essential role of Jun family transcription factors in PU.1 knockdown-induced leukemic stem cells
Steidl, Ulrich
Rosenbauer, Frank
Verhaak, Roel G. W.
Gu, Xuesong
Ebralidze, Alexander
Otu, Hasan H.
Klippel, Steffen
Steidl, Christian
Bruns, Ingmar
Costa, Daniel B.
Wagner, Katharina
Aivado, Manuel
Kobbe, Guido
Valk, Peter J. M.
Passegue, Emmanuelle
Libermann, Towia A.
Delwel, Ruud
Tenen, Daniel G.
[J]. NATURE GENETICS, 2006, 38 (11) : 1269 - 1277

← 1 2 3 4 →