Gnarley1 is a dominant mutation in the knox4 homeobox gene affecting cell shape and identity

被引:68
作者
Foster, T
Yamaguchi, J
Wong, BC
Veit, B
Hake, S [1 ]
机构
[1] Univ Calif Berkeley, Dept Plant & Microbial Sci, Berkeley, CA 94720 USA
[2] Massey Univ, Inst Mol Biosci, Palmerston North, New Zealand
[3] ARS, Ctr Plant Gene Express, USDA, Albany, CA 94710 USA
关键词
D O I
10.1105/tpc.11.7.1239
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Maize leaves have a stereotypical pattern of cell types organized into discrete domains. These domains are altered by mutations in knotted1 (kn1) and knox (for kn1-like homeobox) genes. Gnarley (Gn1) is a dominant maize mutant that exhibits many of the phenotypic characteristics of the kn1 family of mutants. Gn1 is unique because it changes parameters of eel growth in the basal-most region of the leaf, the sheath, resulting in dramatically altered sheath morphology. The strongly expressive allele Gn1-R also gives rise to a floral phenotype in which ectopic carpels form. Introgression studies showed that the severity of the Gn1-conferred phenotype is strongly influenced by genetic background. Gn1 maps to knox4, and knox4 is ectopically expressed in plants with the Gn1-conferred phenotype. Immunolocalization experiments showed that the KNOX protein accumulates at the base of Gn1 leaves in a pattern that is spatially and temporally correlated with appearance of the mutant phenotype. We further demonstrate that Gn1 is knox4 by correlating loss of the mutant phenotype with insertion of a Mutator transposon into knox4.
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页码:1239 / 1252
页数:14
相关论文
共 62 条
[61]   DUPLICATED CHROMOSOME SEGMENTS IN MAIZE (ZEA-MAYS-L) - FURTHER EVIDENCE FROM HEXOKINASE ISOZYMES [J].
WENDEL, JF ;
STUBER, CW ;
EDWARDS, MD ;
GOODMAN, MM .
THEORETICAL AND APPLIED GENETICS, 1986, 72 (02) :178-185
[62]  
WilliamsCarrier RE, 1997, DEVELOPMENT, V124, P3737