Caspase recruitment domain protein 6 is a microtubule-interacting protein that positively modulates NF-κB activation

被引:49
作者
Dufner, A
Pownall, S
Mak, TW [1 ]
机构
[1] Campbell Family Inst Breast Canc Res, Toronto, ON M5G 2C1, Canada
[2] Ontario Canc Inst, Toronto, ON M5G 2C1, Canada
关键词
signal transduction; immunity; receptor-interacting protein kinases;
D O I
10.1073/pnas.0510380103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Proteins containing a caspase recruitment domain (CARD) play pivotal roles in signal transduction leading to apoptosis and NF-kappa B activation and inflammation. Here we identify and characterize human and mouse CARD protein 6 (CARD6), CARD-containing proteins of unique structure. CARD6 associates with microtubules and interacts with receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein kinase (RICK), a CARD-containing member of the RIP family of protein kinases. These kinases are involved in multiple NF-kappa B signaling pathways important for innate and adaptive immune responses. Surprisingly, the CARDs of CARD6 and RICK were not required for their interaction; instead, mutational analysis revealed that the CARD of CARD6 negatively controls the association of these molecules. CARD6 also binds to RIP1, a RIP kinase homologue that lacks a CARD but contains a C-terminal death domain. Coexpression of RICK targets CARD6 to aggresomes via a mechanism that requires the CARD of RICK. Importantly, CARD6 expression has a synergistic effect on NF-kappa B activation induced by several independent signal transduction pathways. In summary, our results indicate that CARD6 is a regulator of NF-kappa B activation that modulates the functions of RIP kinase family members.
引用
收藏
页码:988 / 993
页数:6
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